Integration of plasmids into the bacteriophage T4 genome.

Published

Journal Article

We have analyzed the integration of plasmids into the bacteriophage T4 genome via homologous recombination. As judged by genetic selection for a plasmid-borne marker, a mutation in phage gene uvsX or uvsY essentially blocked the integration of a plasmid with homology to the T4 genome but no phage replication origin (non-origin plasmid). The strict requirement for these two proteins suggests that plasmid integration can proceed via a strand-invasion reaction similar to that catalyzed in vitro by the T4-encoded strand-exchange protein (UvsX) in concert with UvsY and gp32. In contrast to the results with the non-origin plasmid, a mutation in uvsX or uvsY reduced the integration of a T4 replication origin-containing plasmid by only 3-10-fold. These results suggest that the origin-containing plasmid integrates by both the UvsXY-dependent pathway used by the non-origin plasmid and by a UvsXY-independent pathway. The origin-containing plasmid integrated into the phage genome during a uvsX- or uvsY-mutant infection of a recA-mutant host, and therefore origin-dependent integration can occur in the absence of both phage- and host-encoded strand-exchange proteins (UvsX and RecA, respectively).

Full Text

Duke Authors

Cited Authors

  • Kreuzer, HW; Kreuzer, KN

Published Date

  • December 1994

Published In

Volume / Issue

  • 138 / 4

Start / End Page

  • 983 - 992

PubMed ID

  • 7896118

Pubmed Central ID

  • 7896118

International Standard Serial Number (ISSN)

  • 0016-6731

Language

  • eng

Conference Location

  • United States