Deletion of the essential gene 24 from the bacteriophage T4 genome.

Published

Journal Article

We deleted the essential gene 24 from the genome of bacteriophage T4. The delta 24 phage is a conditional lethal mutant that can grow only when the host strain supplies the product of gene 24 in trans, or when the phage acquires a functional gene 24 by some type of recombination event. Thus, gene 24 can be used as a selectable marker, for example permitting transposition into the T4 genome and analyses of plasmid-phage recombination [Woodworth and Kreuzer, Mol. Microbiol. 6 (1992) 1289-1296; H.W.E. and K.N.K., manuscript submitted]. We also found that the promoter region of gene 24 allows a low level of autonomous plasmid replication in T4-infected cells, raising the possibility of a previously unrecognized mode of T4 replication initiation.

Full Text

Duke Authors

Cited Authors

  • Engman, HW; Kreuzer, KN

Published Date

  • January 15, 1993

Published In

Volume / Issue

  • 123 / 1

Start / End Page

  • 69 - 74

PubMed ID

  • 8423006

Pubmed Central ID

  • 8423006

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(93)90541-a

Language

  • eng

Conference Location

  • Netherlands