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Deletion of the essential gene 24 from the bacteriophage T4 genome.

Publication ,  Journal Article
Engman, HW; Kreuzer, KN
Published in: Gene
January 15, 1993

We deleted the essential gene 24 from the genome of bacteriophage T4. The delta 24 phage is a conditional lethal mutant that can grow only when the host strain supplies the product of gene 24 in trans, or when the phage acquires a functional gene 24 by some type of recombination event. Thus, gene 24 can be used as a selectable marker, for example permitting transposition into the T4 genome and analyses of plasmid-phage recombination [Woodworth and Kreuzer, Mol. Microbiol. 6 (1992) 1289-1296; H.W.E. and K.N.K., manuscript submitted]. We also found that the promoter region of gene 24 allows a low level of autonomous plasmid replication in T4-infected cells, raising the possibility of a previously unrecognized mode of T4 replication initiation.

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Published In

Gene

DOI

ISSN

0378-1119

Publication Date

January 15, 1993

Volume

123

Issue

1

Start / End Page

69 / 74

Location

Netherlands

Related Subject Headings

  • Restriction Mapping
  • Genes, Viral
  • Gene Deletion
  • Developmental Biology
  • Cloning, Molecular
  • Capsid
  • Bacteriophage T4
  • 3105 Genetics
  • 3102 Bioinformatics and computational biology
  • 3101 Biochemistry and cell biology
 

Citation

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Engman, H. W., & Kreuzer, K. N. (1993). Deletion of the essential gene 24 from the bacteriophage T4 genome. Gene, 123(1), 69–74. https://doi.org/10.1016/0378-1119(93)90541-a
Engman, H. W., and K. N. Kreuzer. “Deletion of the essential gene 24 from the bacteriophage T4 genome.Gene 123, no. 1 (January 15, 1993): 69–74. https://doi.org/10.1016/0378-1119(93)90541-a.
Engman HW, Kreuzer KN. Deletion of the essential gene 24 from the bacteriophage T4 genome. Gene. 1993 Jan 15;123(1):69–74.
Engman, H. W., and K. N. Kreuzer. “Deletion of the essential gene 24 from the bacteriophage T4 genome.Gene, vol. 123, no. 1, Jan. 1993, pp. 69–74. Pubmed, doi:10.1016/0378-1119(93)90541-a.
Engman HW, Kreuzer KN. Deletion of the essential gene 24 from the bacteriophage T4 genome. Gene. 1993 Jan 15;123(1):69–74.
Journal cover image

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

January 15, 1993

Volume

123

Issue

1

Start / End Page

69 / 74

Location

Netherlands

Related Subject Headings

  • Restriction Mapping
  • Genes, Viral
  • Gene Deletion
  • Developmental Biology
  • Cloning, Molecular
  • Capsid
  • Bacteriophage T4
  • 3105 Genetics
  • 3102 Bioinformatics and computational biology
  • 3101 Biochemistry and cell biology