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Deletion analysis of bacteriophage T4 tertiary origins. A promoter sequence is required for a rifampicin-resistant replication origin.

Publication ,  Journal Article
Menkens, AE; Kreuzer, KN
Published in: J Biol Chem
August 15, 1988

The DNA sequence requirements of two T4 tertiary replication origins have been characterized by a deletion analysis of origin-containing plasmids. Maximal replication of each origin-containing plasmid required both an intact gpmotA-dependent middle-mode promoter sequence and approximately 50 base pairs of the downstream region. In contrast, gpmotA-dependent transcription from the origin promoter was found to be independent of the downstream region. The requirement for a promoter element within the tertiary origins is striking, particularly since the replication of tertiary origin-containing plasmids is resistant to the RNA polymerase inhibitor rifampicin.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

August 15, 1988

Volume

263

Issue

23

Start / End Page

11358 / 11365

Location

United States

Related Subject Headings

  • Virus Replication
  • Transcription, Genetic
  • T-Phages
  • Rifampin
  • Promoter Regions, Genetic
  • Plasmids
  • Drug Resistance, Microbial
  • Chromosome Deletion
  • Biochemistry & Molecular Biology
  • Base Sequence
 

Citation

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

August 15, 1988

Volume

263

Issue

23

Start / End Page

11358 / 11365

Location

United States

Related Subject Headings

  • Virus Replication
  • Transcription, Genetic
  • T-Phages
  • Rifampin
  • Promoter Regions, Genetic
  • Plasmids
  • Drug Resistance, Microbial
  • Chromosome Deletion
  • Biochemistry & Molecular Biology
  • Base Sequence