Deletion analysis of bacteriophage T4 tertiary origins. A promoter sequence is required for a rifampicin-resistant replication origin.

Published

Journal Article

The DNA sequence requirements of two T4 tertiary replication origins have been characterized by a deletion analysis of origin-containing plasmids. Maximal replication of each origin-containing plasmid required both an intact gpmotA-dependent middle-mode promoter sequence and approximately 50 base pairs of the downstream region. In contrast, gpmotA-dependent transcription from the origin promoter was found to be independent of the downstream region. The requirement for a promoter element within the tertiary origins is striking, particularly since the replication of tertiary origin-containing plasmids is resistant to the RNA polymerase inhibitor rifampicin.

Full Text

Duke Authors

Cited Authors

  • Menkens, AE; Kreuzer, KN

Published Date

  • August 15, 1988

Published In

Volume / Issue

  • 263 / 23

Start / End Page

  • 11358 - 11365

PubMed ID

  • 3403531

Pubmed Central ID

  • 3403531

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States