Bacteriophage T4 initiates bidirectional DNA replication through a two-step process.

Journal Article (Journal Article)

Two-dimensional gel analysis of the bacteriophage T4 ori(uvsY) region revealed a novel "comet" on the Y arc. This comet contains simple Y molecules in which the branch points map to the ori(uvsY) transcript region. The comet depends on the the origin and DNA synthesis and is abolished by a mutation that reduces replication without affecting transcription. These results argue that the branched molecules are intermediates in replication initiation. A transcriptional terminator, cloned just downstream of the origin promoter, shortened the tail of the comet. Therefore, the location of the transcript determines the DNA branch points. We conclude that the comet DNA consists of intermediates in which unidirectional replication has been triggered by priming from the RNA of the origin R loop.

Full Text

Duke Authors

Cited Authors

  • Belanger, KG; Kreuzer, KN

Published Date

  • November 1998

Published In

Volume / Issue

  • 2 / 5

Start / End Page

  • 693 - 701

PubMed ID

  • 9844641

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/s1097-2765(00)80167-7


  • eng

Conference Location

  • United States