Apparent suicide inactivation of human lymphoblast S-adenosylhomocysteine hydrolase by 2'-deoxyadenosine and adenine arabinoside. A basis for direct toxic effects of analogs of adenosine.

Journal Article (Journal Article)

Adenosylhomocysteine hydrolase from human lymphoblasts binds 2'-deoxy[3H]adenosine tightly. Binding is associated with time-dependent, saturable, irreversible inactivation of catalytic activity which occurs with first order kinetics, suggesting "suicide" inactivation. Adenine arabinoside produces similar inactivation but is more potent. These results suggest a basis for a heretofore unrecognized mechanism of action for these and other analogs of adenosine, in which toxicity results from actions of the nucleosides themselves, rather than from nucleotides to which they may be converted.

Full Text

Duke Authors

Cited Authors

  • Hershfield, MS

Published Date

  • January 10, 1979

Published In

Volume / Issue

  • 254 / 1

Start / End Page

  • 22 - 25

PubMed ID

  • 309884

International Standard Serial Number (ISSN)

  • 0021-9258


  • eng

Conference Location

  • United States