A radiochemical-high-performance liquid chromatographic assay for urate oxidase in human plasma.

Journal Article (Journal Article)

Polyethylene glycol-modified urate oxidase (PEG-uricase) holds promise as a hypouricemic agent for treating gout and as an adjunct to cytolytic therapy of hematologic malignancies. Spectrophotometric assays of urate oxidase are not sensitive enough for pharmacokinetic evaluation of PEG-uricase in clinical trials. We have therefore developed a more sensitive radiochemical-HPLC assay for urate oxidase activity in untreated plasma, in which 14C in urate and in the reaction product, allantoin, is monitored in the uv detector effluent with a flow-through scintillation counter. The assay is linear with amount of enzyme and time of incubation and can detect less than 1 x 10(-5) U/ml uricase in plasma. The assay accounts for plasma samples of widely differing urate content.

Full Text

Duke Authors

Cited Authors

  • Greenberg, ML; Hershfield, MS

Published Date

  • February 1, 1989

Published In

Volume / Issue

  • 176 / 2

Start / End Page

  • 290 - 293

PubMed ID

  • 2742121

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(89)90311-4


  • eng

Conference Location

  • United States