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Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine.

Publication ,  Journal Article
Hebert, ME; Greenberg, ML; Chaffee, S; Gravatt, L; Hershfield, MS; Elion, GB; Kurtzberg, J
Published in: Transplantation
October 1991

Arabinosylguanine (araG) is a nucleoside analog that is rapidly converted by cells of the T lymphoid lineage to its corresponding arabinosylguanine nucleotide triphosphate, resulting in inhibition of DNA synthesis and selective in vitro toxicity to T lymphoblastoid cell lines as well as to freshly isolated leukemia cells from patients with T cell acute lymphoblastic leukemia. In this report, we demonstrate that araG is an effective agent to use for chemoseparation of malignant T lymphoblasts from human bone marrow. When freshly isolated human T leukemia cells or T lymphoblastoid cells were treated with 100 microM araG for 18 hr, up to 6 logs of clonogenic T cells could be eliminated without appreciable toxicity to the normal myeloid, erythroid, and megakaryocytoid clonal progenitor cells. We discuss the use of this agent in ex vivo elimination of residual malignant T cells from marrow of patients requiring myeloablative chemotherapy with autologous bone marrow rescue.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

October 1991

Volume

52

Issue

4

Start / End Page

634 / 640

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • Surgery
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma
  • Pentostatin
  • Leukemia-Lymphoma, Adult T-Cell
  • Humans
  • Guanosine Triphosphate
  • Erythroid Precursor Cells
  • Clone Cells
 

Citation

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Hebert, M. E., Greenberg, M. L., Chaffee, S., Gravatt, L., Hershfield, M. S., Elion, G. B., & Kurtzberg, J. (1991). Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine. Transplantation, 52(4), 634–640. https://doi.org/10.1097/00007890-199110000-00011
Hebert, M. E., M. L. Greenberg, S. Chaffee, L. Gravatt, M. S. Hershfield, G. B. Elion, and J. Kurtzberg. “Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine.Transplantation 52, no. 4 (October 1991): 634–40. https://doi.org/10.1097/00007890-199110000-00011.
Hebert ME, Greenberg ML, Chaffee S, Gravatt L, Hershfield MS, Elion GB, et al. Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine. Transplantation. 1991 Oct;52(4):634–40.
Hebert, M. E., et al. “Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine.Transplantation, vol. 52, no. 4, Oct. 1991, pp. 634–40. Pubmed, doi:10.1097/00007890-199110000-00011.
Hebert ME, Greenberg ML, Chaffee S, Gravatt L, Hershfield MS, Elion GB, Kurtzberg J. Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine. Transplantation. 1991 Oct;52(4):634–640.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

October 1991

Volume

52

Issue

4

Start / End Page

634 / 640

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • Surgery
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma
  • Pentostatin
  • Leukemia-Lymphoma, Adult T-Cell
  • Humans
  • Guanosine Triphosphate
  • Erythroid Precursor Cells
  • Clone Cells