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A human recombinant Fab identifies a human immunodeficiency virus type 1-induced conformational change in cell surface-expressed CD4.

Publication ,  Journal Article
Bachelder, RE; Bilancieri, J; Lin, W; Letvin, NL
Published in: Journal of virology
September 1995

To explore the role of the CD4 molecule in human immunodeficiency virus (HIV) infection following initial virus-CD4 binding, we have characterized CD4-specific antibodies raised by immunizing an HIV-1-infected human with human recombinant soluble CD4 (rsCD4). Fabs were selected from a human recombinant Fab library constructed from the bone marrow of this immunized individual. Here, we describe a human rsCD4-specific recombinant Fab clone selected by panning the library over complexes of human rsCD4 and recombinant HIV-1 envelope protein. While this Fab does not bind to CD4-positive T-cell lines or to human T lymphocytes, it recognizes cell surface-expressed CD4 following the incubation of these cells with a recombinant form of HIV-1 gp120 or with HIV-1 virions. The Fab is not HIV-1 envelope specific, since it does not bind to recombinant gp120 or to native cell surface-expressed HIV-1 envelope proteins. As confirmation of its CD4 specificity, we show that this Fab immunoprecipitates a 55-kDa protein, corresponding to the molecular mass of cellular CD4, from an H9 cell lysate. The specificity of this human Fab provides evidence for a virus-induced conformational change in cell surface-expressed on CD4. The characterization of this altered CD4 conformation and its effects on the host cell will be important in defining postbinding events in HIV infection.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

September 1995

Volume

69

Issue

9

Start / End Page

5734 / 5742

Related Subject Headings

  • Virology
  • T-Lymphocytes
  • Recombinant Proteins
  • Receptors, Virus
  • RNA, Messenger
  • Protein Conformation
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Kidney
  • Immunoglobulin lambda-Chains
 

Citation

APA
Chicago
ICMJE
MLA
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Bachelder, R. E., Bilancieri, J., Lin, W., & Letvin, N. L. (1995). A human recombinant Fab identifies a human immunodeficiency virus type 1-induced conformational change in cell surface-expressed CD4. Journal of Virology, 69(9), 5734–5742. https://doi.org/10.1128/jvi.69.9.5734-5742.1995
Bachelder, R. E., J. Bilancieri, W. Lin, and N. L. Letvin. “A human recombinant Fab identifies a human immunodeficiency virus type 1-induced conformational change in cell surface-expressed CD4.Journal of Virology 69, no. 9 (September 1995): 5734–42. https://doi.org/10.1128/jvi.69.9.5734-5742.1995.
Bachelder RE, Bilancieri J, Lin W, Letvin NL. A human recombinant Fab identifies a human immunodeficiency virus type 1-induced conformational change in cell surface-expressed CD4. Journal of virology. 1995 Sep;69(9):5734–42.
Bachelder, R. E., et al. “A human recombinant Fab identifies a human immunodeficiency virus type 1-induced conformational change in cell surface-expressed CD4.Journal of Virology, vol. 69, no. 9, Sept. 1995, pp. 5734–42. Epmc, doi:10.1128/jvi.69.9.5734-5742.1995.
Bachelder RE, Bilancieri J, Lin W, Letvin NL. A human recombinant Fab identifies a human immunodeficiency virus type 1-induced conformational change in cell surface-expressed CD4. Journal of virology. 1995 Sep;69(9):5734–5742.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

September 1995

Volume

69

Issue

9

Start / End Page

5734 / 5742

Related Subject Headings

  • Virology
  • T-Lymphocytes
  • Recombinant Proteins
  • Receptors, Virus
  • RNA, Messenger
  • Protein Conformation
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Kidney
  • Immunoglobulin lambda-Chains