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Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor.

Publication ,  Journal Article
Saso, L; Leone, MG; Sorrentino, C; Giacomelli, S; Silvestrini, B; Grima, J; Li, JC; Samy, E; Mruk, D; Cheng, CY
Published in: Biochemistry and molecular biology international
November 1998

Prostaglandin D synthetase (PGD-S; prostaglandin-H2 D-isomerase, EC 5,3,99,2), a 30 kDa glycoprotein also known as beta-trace protein that catalyzes the formation of prostaglandin D2 (PGD2) from PGH2, was purified to apparent homogeneity from human cerebrospinal fluid (CSF) using a two-step procedure involving HPLC on a Vydac C8 reversed-phase column and high performance electrophoresis chromatography (HPEC) using a 10% T SDS-polyacrylamide gel. The purity of PGD-S isolated from CSF was confirmed by silver stained SDS-polyacrylamide gel and direct protein microsequencing (NH2-APEAQVSVQPNFQ). A highly specific polyclonal antibody was prepared against this protein for immunoassay development. Using an ELISA, it was found that the concentration of PGD-S in CSF did not alter significantly in different pathological conditions of the central nervous system (CNS). These include dementia (n = 9), hydrocephalus (n = 4), neuropathy (n = 11), optic neuritis (n = 4), multiple sclerosis (n = 11), and demyelinating syndrome (n = 11), when compared to normal individuals (n = 12); however, the level of PGD-S in the CSF obtained from patients with brain tumor (n = 11), was reduced by as much as 2-fold when compared to control samples (n = 12) illustrating PGD-S is a potentially useful marker for brain tumor.

Duke Scholars

Published In

Biochemistry and molecular biology international

ISSN

1039-9712

Publication Date

November 1998

Volume

46

Issue

4

Start / End Page

643 / 656

Location

australia

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1101 Medical Biochemistry and Metabolomics
  • 0604 Genetics
  • 0601 Biochemistry and Cell Biology
 

Citation

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Saso, L., Leone, M. G., Sorrentino, C., Giacomelli, S., Silvestrini, B., Grima, J., … Cheng, C. Y. (1998). Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor. Biochemistry and Molecular Biology International, 46(4), 643–656.
Saso, L., M. G. Leone, C. Sorrentino, S. Giacomelli, B. Silvestrini, J. Grima, J. C. Li, E. Samy, D. Mruk, and C. Y. Cheng. “Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor.Biochemistry and Molecular Biology International 46, no. 4 (November 1998): 643–56.
Saso L, Leone MG, Sorrentino C, Giacomelli S, Silvestrini B, Grima J, et al. Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor. Biochemistry and molecular biology international. 1998 Nov;46(4):643–56.
Saso, L., et al. “Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor.Biochemistry and Molecular Biology International, vol. 46, no. 4, Nov. 1998, pp. 643–56.
Saso L, Leone MG, Sorrentino C, Giacomelli S, Silvestrini B, Grima J, Li JC, Samy E, Mruk D, Cheng CY. Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor. Biochemistry and molecular biology international. 1998 Nov;46(4):643–656.

Published In

Biochemistry and molecular biology international

ISSN

1039-9712

Publication Date

November 1998

Volume

46

Issue

4

Start / End Page

643 / 656

Location

australia

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1101 Medical Biochemistry and Metabolomics
  • 0604 Genetics
  • 0601 Biochemistry and Cell Biology