The effects of periurethral muscle-derived stem cell injection on leak point pressure in a rat model of stress urinary incontinence.

Journal Article (Journal Article)

Our goal was to determine whether periurethral injection of allogenic muscle-derived stem cells (MDSC) could increase the leak point pressure (LPP) in a denervated female rat model of stress urinary incontinence. Cells isolated from the gastrocnemius muscle of normal female rats were purified for a myogenic population by the preplate technique. Three experimental groups were established: a control group (C) had a sham operation without injections; a sciatic nerve transection group (D) had periurethral saline injections; and a sciatic nerve transsection group had periurethral MDSC injections (M). One week following treatment the LPP of groups C, D and M were 25.2+/-1.9 cmH(2)O, 28.6+/-0.8 cmH(2)O and 36.7+/-2.3 cmH(2)O, respectively. At 4 weeks the LPP of groups C, D and M were 25.8+/-2.5 cmH(2)O, 18.6+/-5.2 cmH(2)O and 44.1+/-6.6 cmH(2)O, respectively. Allogenic MDSC significantly improved the LPP in sciatic nerve-transected animals after both 1 and 4 weeks compared to denervated animals injected with saline.

Full Text

Duke Authors

Cited Authors

  • Lee, JY; Cannon, TW; Pruchnic, R; Fraser, MO; Huard, J; Chancellor, MB

Published Date

  • February 2003

Published In

  • Int Urogynecol J Pelvic Floor Dysfunct

Volume / Issue

  • 14 / 1

Start / End Page

  • 31 - 37

PubMed ID

  • 12601514

Digital Object Identifier (DOI)

  • 10.1007/s00192-002-1004-5


  • eng

Conference Location

  • England