In utero hematopoietic stem cell transplants prolong survival of postnatal kidney transplantation in monkeys.

Published

Journal Article

The authors hypothesized that in utero transplantation of T-cell-depleted paternal marrow into rhesus monkey fetuses would induce tolerance to postnatal kidney grafts from the marrow donor. T-cell-depleted paternal bone marrow was transplanted intraperitoneally into two female fetal rhesus monkeys at 61 +/- 1 days' gestation. Chimeric monkeys (n = 2) received kidney transplants from paternal donors. Control monkeys (n = 2) underwent kidney transplants without prior in utero stem cell transplants. Both chimeric monkeys demonstrated low level (<0.1% donor cells) engraftment in the bone marrow and peripheral blood using the polymerase chain reaction assay for the Y chromosome. The mixed lymphocyte reaction demonstrated hyporeactivity to the donor. Control animals demonstrated severe acute rejection and graft failure 1 week posttransplant. The first chimeric monkey had no significant clinical or sonographic evidence of renal failure until 7 weeks after the transplant. Biopsy findings showed mild rejection 1 week postoperatively, but rejection did not significantly progress until 5 weeks later. The second chimeric monkey had no significant clinical or sonographic changes for 4 weeks, but evidence of moderate rejection was seen on biopsy results. This monkey was given a 10-week course of immunosuppression, and had no clinical or sonographic renal deterioration, although biopsy results showed chronic rejection that was confirmed when electively euthanized 8 months later. Our data suggest that in utero transplantation of hematopoietic stem cells can increase the survival of a kidney allograft in the rhesus monkey.

Full Text

Duke Authors

Cited Authors

  • Mychaliska, GB; Rice, HE; Tarantal, AF; Stock, PG; Capper, J; Garovoy, MR; Olson, JL; Cowan, MJ; Harrison, MR

Published Date

  • July 1997

Published In

Volume / Issue

  • 32 / 7

Start / End Page

  • 976 - 981

PubMed ID

  • 9247216

Pubmed Central ID

  • 9247216

International Standard Serial Number (ISSN)

  • 0022-3468

Language

  • eng

Conference Location

  • United States