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Molecular detection of prostate epithelial cells from the surgical field and peripheral circulation during radical prostatectomy.

Publication ,  Journal Article
Oefelein, MG; Kaul, K; Herz, B; Blum, MD; Holland, JM; Keeler, TC; Cook, WA; Ignatoff, JM
Published in: The Journal of urology
January 1996

PURPOSE: Prostate cancer progression despite organ confined pathological assessment has been reported in a variable number of men after radical retropubic prostatectomy. To study this phenomenon, we used the prostate specific antigen (PSA) reverse transcriptase-polymerase chain reaction assay. MATERIALS AND METHODS: We prospectively assayed the peripheral venous blood before, during and after surgical manipulation as well as the intraoperative field blood for PSA reverse transcriptase-polymerase chain reaction-positive cells in 22 men undergoing radical retropubic prostatectomy. RESULTS: PSA reverse transcriptase-polymerase chain reaction-positive cells were identified in 20 of the 22 operative field samples (91%) and 4 of 16 (25%) had evidence of intraoperative hematogenous dissemination (p = 0.046). No significant association was identified among Gleason score, pathological stage and the PSA reverse transcriptase-polymerase chain reaction result. CONCLUSIONS: Our results suggest that tumor cell spillage and less frequently hematogenous dissemination may be associated with operative manipulation of the prostate during radical retropubic prostatectomy and may potentially represent mechanisms of failure after radical retropubic prostatectomy.

Duke Scholars

Published In

The Journal of urology

ISSN

0022-5347

Publication Date

January 1996

Volume

155

Issue

1

Start / End Page

238 / 242

Location

united states

Related Subject Headings

  • Urology & Nephrology
  • 1103 Clinical Sciences
 

Citation

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Oefelein, M. G., Kaul, K., Herz, B., Blum, M. D., Holland, J. M., Keeler, T. C., … Ignatoff, J. M. (1996). Molecular detection of prostate epithelial cells from the surgical field and peripheral circulation during radical prostatectomy. The Journal of Urology, 155(1), 238–242.
Oefelein, M. G., K. Kaul, B. Herz, M. D. Blum, J. M. Holland, T. C. Keeler, W. A. Cook, and J. M. Ignatoff. “Molecular detection of prostate epithelial cells from the surgical field and peripheral circulation during radical prostatectomy.The Journal of Urology 155, no. 1 (January 1996): 238–42.
Oefelein MG, Kaul K, Herz B, Blum MD, Holland JM, Keeler TC, et al. Molecular detection of prostate epithelial cells from the surgical field and peripheral circulation during radical prostatectomy. The Journal of urology. 1996 Jan;155(1):238–42.
Oefelein, M. G., et al. “Molecular detection of prostate epithelial cells from the surgical field and peripheral circulation during radical prostatectomy.The Journal of Urology, vol. 155, no. 1, Jan. 1996, pp. 238–42.
Oefelein MG, Kaul K, Herz B, Blum MD, Holland JM, Keeler TC, Cook WA, Ignatoff JM. Molecular detection of prostate epithelial cells from the surgical field and peripheral circulation during radical prostatectomy. The Journal of urology. 1996 Jan;155(1):238–242.
Journal cover image

Published In

The Journal of urology

ISSN

0022-5347

Publication Date

January 1996

Volume

155

Issue

1

Start / End Page

238 / 242

Location

united states

Related Subject Headings

  • Urology & Nephrology
  • 1103 Clinical Sciences