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Establishment and characterization of a new human prostatic carcinoma cell line (DuPro-1).

Publication ,  Journal Article
Gingrich, JR; Tucker, JA; Walther, PJ; Day, JW; Poulton, SH; Webb, KS
Published in: J Urol
September 1991

A new human prostate adenocarcinoma cell line (DuPro-1) has been established from the athymic nude mouse supported xenograft DU5683. This was accomplished by embedding dispersed xenograft cells in 0.1 by 5.0 cm. spaghetti-like strands of Basement Membrane MATRIGEL [BMM (Collaborative Research, Inc.)], a unique technique facilitating the transition to tissue culture. Now passed over 30 times, the cells display anchorage and serum concentration independent growth with a doubling time of 22 to 24 hours. Cells exhibit pronounced morphological differences when grown on BMM coated culture dishes, assuming a pseudoglandular configuration, in contrast to typical homogeneous monolayer growth on plastic culture dishes. Light and electron microscopy show cohesive sheets of anaplastic epithelial cells, consistent with prostate carcinoma. Karyotypic analysis revealed all human chromosomes, near tetraploidy, 10 to 12 markers, and 3 to 4 X chromosomes, without a Y chromosome. Cells injected s.c. or embedded in BMM and implanted in the subrenal capsule space are equally tumorigenic in male and female athymic mice, suggesting that DuPro-1 cells are hormonally insensitive. Embedding cells in BMM may be useful in developing other tissue culture cell lines from neoplasms difficult to initiate in vitro. DuPro-1 should provide a valuable means to study the biology, immunology, and chemosensitivity of human prostate cancer.

Duke Scholars

Published In

J Urol

DOI

ISSN

0022-5347

Publication Date

September 1991

Volume

146

Issue

3

Start / End Page

915 / 919

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Neoplasm Transplantation
  • Middle Aged
  • Mice, Nude
  • Mice
  • Male
  • Karyotyping
  • Humans
 

Citation

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Chicago
ICMJE
MLA
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Gingrich, J. R., Tucker, J. A., Walther, P. J., Day, J. W., Poulton, S. H., & Webb, K. S. (1991). Establishment and characterization of a new human prostatic carcinoma cell line (DuPro-1). J Urol, 146(3), 915–919. https://doi.org/10.1016/s0022-5347(17)37960-0
Gingrich, J. R., J. A. Tucker, P. J. Walther, J. W. Day, S. H. Poulton, and K. S. Webb. “Establishment and characterization of a new human prostatic carcinoma cell line (DuPro-1).J Urol 146, no. 3 (September 1991): 915–19. https://doi.org/10.1016/s0022-5347(17)37960-0.
Gingrich JR, Tucker JA, Walther PJ, Day JW, Poulton SH, Webb KS. Establishment and characterization of a new human prostatic carcinoma cell line (DuPro-1). J Urol. 1991 Sep;146(3):915–9.
Gingrich, J. R., et al. “Establishment and characterization of a new human prostatic carcinoma cell line (DuPro-1).J Urol, vol. 146, no. 3, Sept. 1991, pp. 915–19. Pubmed, doi:10.1016/s0022-5347(17)37960-0.
Gingrich JR, Tucker JA, Walther PJ, Day JW, Poulton SH, Webb KS. Establishment and characterization of a new human prostatic carcinoma cell line (DuPro-1). J Urol. 1991 Sep;146(3):915–919.
Journal cover image

Published In

J Urol

DOI

ISSN

0022-5347

Publication Date

September 1991

Volume

146

Issue

3

Start / End Page

915 / 919

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Neoplasm Transplantation
  • Middle Aged
  • Mice, Nude
  • Mice
  • Male
  • Karyotyping
  • Humans