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Characterization of a new model of human prostatic cancer: the multicellular tumor spheroid.

Publication ,  Journal Article
Donaldson, JT; Tucker, JA; Keane, TE; Walther, PJ; Webb, KS
Published in: Int J Cancer
August 15, 1990

Multicellular tumor spheroids (MTS) provide a closer in vitro correlate to in vivo malignancy than do conventional monolayer cultures; while simulating many parameters of in vivo growth, MTS systems provide those perquisites (i.e., experimental control, economy, expediency) associated with in vitro evaluation of preclinical therapeutic strategies. For these reasons, we exploited the proclivity of the highly metastatic human prostatic carcinoma subline I-LN-PC3-IA to spontaneously assume a spheroid morphology under routine culture conditions. I-LN spheroids demonstrate salient features described in other spheroid systems and exhibit histologic characteristics of human prostate carcinoma. Cells encompassed in the I-LN spheroid format demonstrated functional divergence from their monolayer counterparts with respect to immunoreactivity for prostatic acid phosphatase, positional dependence of prostate-restricted p40 antigen expression, and chemotherapeutic drug response. This new in vitro-in vivo transition model of human prostatic carcinoma should provide a valuable in vitro context to expediently evaluate in vivo correlates of oncolytic protocols on a malignancy that remains refractive to therapy.

Duke Scholars

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

August 15, 1990

Volume

46

Issue

2

Start / End Page

238 / 244

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Thymidine
  • Prostatic Neoplasms
  • Prostate
  • Oxidation-Reduction
  • Oncology & Carcinogenesis
  • Models, Biological
  • Microscopy, Electron
  • Male
  • Immunoenzyme Techniques
 

Citation

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MLA
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Donaldson, J. T., Tucker, J. A., Keane, T. E., Walther, P. J., & Webb, K. S. (1990). Characterization of a new model of human prostatic cancer: the multicellular tumor spheroid. Int J Cancer, 46(2), 238–244. https://doi.org/10.1002/ijc.2910460216
Donaldson, J. T., J. A. Tucker, T. E. Keane, P. J. Walther, and K. S. Webb. “Characterization of a new model of human prostatic cancer: the multicellular tumor spheroid.Int J Cancer 46, no. 2 (August 15, 1990): 238–44. https://doi.org/10.1002/ijc.2910460216.
Donaldson JT, Tucker JA, Keane TE, Walther PJ, Webb KS. Characterization of a new model of human prostatic cancer: the multicellular tumor spheroid. Int J Cancer. 1990 Aug 15;46(2):238–44.
Donaldson, J. T., et al. “Characterization of a new model of human prostatic cancer: the multicellular tumor spheroid.Int J Cancer, vol. 46, no. 2, Aug. 1990, pp. 238–44. Pubmed, doi:10.1002/ijc.2910460216.
Donaldson JT, Tucker JA, Keane TE, Walther PJ, Webb KS. Characterization of a new model of human prostatic cancer: the multicellular tumor spheroid. Int J Cancer. 1990 Aug 15;46(2):238–244.
Journal cover image

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

August 15, 1990

Volume

46

Issue

2

Start / End Page

238 / 244

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Thymidine
  • Prostatic Neoplasms
  • Prostate
  • Oxidation-Reduction
  • Oncology & Carcinogenesis
  • Models, Biological
  • Microscopy, Electron
  • Male
  • Immunoenzyme Techniques