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p53 gene alterations in human prostate carcinoma.

Publication ,  Journal Article
Effert, PJ; McCoy, RH; Walther, PJ; Liu, ET
Published in: J Urol
July 1993

Alterations of the p53 gene are among the most frequent genetic lesions in a variety of human malignancies. Their role in prostate cancer is, however, unclear. We have analyzed 10 primary and two metastatic human prostate carcinomas as well as 3 prostate cancer cell lines for mutations of the p53-gene. Using single strand conformational polymorphism analysis (SSCP) and direct sequencing of the polymerase chain reaction (PCR) products, p53 mutations were detected in 1 of 10 primary prostate cancers. By contrast, 1 of 2 metastatic tumors and all 3 prostate cancer cell lines (derived from metastases) were found to contain a mutant p53 gene. Thus, our data suggest that alterations of the p53 gene at the mutational "hot spots" appear to occur infrequently in primary human prostate cancer, but may emerge in later stages of tumor progression. Furthermore, we confirm that loss of heterozygosity at a locus telomeric to p53, but not including p53, is associated with metastatic progression in 1 case.

Duke Scholars

Published In

J Urol

DOI

ISSN

0022-5347

Publication Date

July 1993

Volume

150

Issue

1

Start / End Page

257 / 261

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Mutation
  • Male
  • Lymphatic Metastasis
  • Humans
  • Genes, p53
 

Citation

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Effert, P. J., McCoy, R. H., Walther, P. J., & Liu, E. T. (1993). p53 gene alterations in human prostate carcinoma. J Urol, 150(1), 257–261. https://doi.org/10.1016/s0022-5347(17)35458-7
Effert, P. J., R. H. McCoy, P. J. Walther, and E. T. Liu. “p53 gene alterations in human prostate carcinoma.J Urol 150, no. 1 (July 1993): 257–61. https://doi.org/10.1016/s0022-5347(17)35458-7.
Effert PJ, McCoy RH, Walther PJ, Liu ET. p53 gene alterations in human prostate carcinoma. J Urol. 1993 Jul;150(1):257–61.
Effert, P. J., et al. “p53 gene alterations in human prostate carcinoma.J Urol, vol. 150, no. 1, July 1993, pp. 257–61. Pubmed, doi:10.1016/s0022-5347(17)35458-7.
Effert PJ, McCoy RH, Walther PJ, Liu ET. p53 gene alterations in human prostate carcinoma. J Urol. 1993 Jul;150(1):257–261.
Journal cover image

Published In

J Urol

DOI

ISSN

0022-5347

Publication Date

July 1993

Volume

150

Issue

1

Start / End Page

257 / 261

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Mutation
  • Male
  • Lymphatic Metastasis
  • Humans
  • Genes, p53