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An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors.

Publication ,  Journal Article
Shrivastav, S; Bonar, RA; Stone, KR; Paulson, DF
Published in: Cancer Res
December 1980

An in vitro assay was developed to measure the chemotherapeutic drug susceptibility of cells from human tumors. The assay utilized live cells, freshly isolated from tumor tissue, which were incubated for a short period in vitro. The drug-induced inhibition of incorporation of radiolabeled precursor into DNA, RNA, and protein was measured. The assay is sensitive to concentrations of chemotherapeutic drugs in the therapeutic range and is reproducible when tested with replicates of the same tumor cell population.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

December 1980

Volume

40

Issue

12

Start / End Page

4438 / 4442

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mice, Nude
  • Mice
  • Humans
  • Drug Evaluation, Preclinical
  • Doxorubicin
  • Dose-Response Relationship, Drug
  • DNA, Neoplasm
  • Cyclophosphamide
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shrivastav, S., Bonar, R. A., Stone, K. R., & Paulson, D. F. (1980). An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors. Cancer Res, 40(12), 4438–4442.
Shrivastav, S., R. A. Bonar, K. R. Stone, and D. F. Paulson. “An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors.Cancer Res 40, no. 12 (December 1980): 4438–42.
Shrivastav S, Bonar RA, Stone KR, Paulson DF. An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors. Cancer Res. 1980 Dec;40(12):4438–42.
Shrivastav, S., et al. “An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors.Cancer Res, vol. 40, no. 12, Dec. 1980, pp. 4438–42.
Shrivastav S, Bonar RA, Stone KR, Paulson DF. An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors. Cancer Res. 1980 Dec;40(12):4438–4442.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

December 1980

Volume

40

Issue

12

Start / End Page

4438 / 4442

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mice, Nude
  • Mice
  • Humans
  • Drug Evaluation, Preclinical
  • Doxorubicin
  • Dose-Response Relationship, Drug
  • DNA, Neoplasm
  • Cyclophosphamide