Development and application of basic research techniques in bladder cancer research.

Journal Article (Journal Article;Review)

The growth of transitional epithelial cells with different growth media and growth supports was examined. Sephadex G-10, Bio-Gel P-20, Bio-Glas-1000, DEAE-Sephadex A-50, DEAE-cellulose, CM-Sephadex C-50, acid-soluble collagen, and immobilized collagen fibers were used to enhance plating efficiency. Acid-soluble collagen layers optimally increased the plating efficiency of primary cultures of bladder carcinoma. Media alterations with serial combinations of fetal calf, newborn calf, calf, bovine, and bull serum with minimum essential medium, Roswell Park Memorial Institute Tissue Culture Medium 1640, Connaught Medical Research Laboratories Medium 1066, Medium 199, Grand Island Biological, National Cancer Tissue Culture 135, 1415, McCoy's 5A, and National Cancer Institute medium were established. No promotion of cell division was noted with any one of these basic medium formulations.

Full Text

Duke Authors

Cited Authors

  • Paulson, DF; Stone, KR; Mickey, DD; Bonar, RA; Wunderli, H

Published Date

  • August 1977

Published In

Volume / Issue

  • 37 / 8 Pt 2

Start / End Page

  • 2969 - 2973

PubMed ID

  • 326397

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States