Correlation of apparent molecular weight and antigenicity of viral proteins: an SDS-page separation followed by acrylamide-agarose electrophoresis and immunoprecipitation.
A simple method is described which combines a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE) in the first demension with a second electrophoresis, at right angles to the first, into an agarose matrix. The proteins, separated by SDS-PAGE, are exposed to appropriate antisera after the second stage electrophoresis and immunoprecipitates form in the agarose corresponding to the relative electrophoretic mobilities of proteins in the first stage SDS-PAGE separation. The method thus provides a simple, reproducible means for correlating antigenicity with apparent molecular weight of proteins. The technique is qualtitative, but requires smaller quantities of antisera than more conventional immunoelectrophoretic methods such as rocket electrophoresis.
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Related Subject Headings
- Viral Proteins
- Sodium Dodecyl Sulfate
- Molecular Weight
- Immunology
- Immunologic Techniques
- Friend murine leukemia virus
- Electrophoresis, Polyacrylamide Gel
- Electrophoresis, Agar Gel
- Chemical Precipitation
- Avian myeloblastosis virus
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Viral Proteins
- Sodium Dodecyl Sulfate
- Molecular Weight
- Immunology
- Immunologic Techniques
- Friend murine leukemia virus
- Electrophoresis, Polyacrylamide Gel
- Electrophoresis, Agar Gel
- Chemical Precipitation
- Avian myeloblastosis virus