Activation of cyclophosphamide for in vitro testing of cell sensitivity.

Journal Article (Journal Article)

Two methods are described for the activation of cyclophosphamide by a liver microsome preparation. These procedures were applicable to an assay in vitro which tests the sensitivity of tumor cells to the drug. Satisfactory results were obtained either by pretreatment of the cyclophosphamide and removal of the microsomes before testing or by the somewhat simpler procedure of mixing drug, microsomes, and test cells for the assay. Microsome treatment of bleomycin gave a smaller increase in activity, and much smaller effects were seen on some other drugs.

Full Text

Duke Authors

Cited Authors

  • Shrivastav, S; Stone, KR; Paulson, DF; Bonar, RA

Published Date

  • December 1, 1980

Published In

Volume / Issue

  • 40 / 12

Start / End Page

  • 4443 - 4445

PubMed ID

  • 7438076

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States