Isolation of a human prostate carcinoma cell line (DU 145).
Journal Article (Journal Article)
A long-term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.
Full Text
Duke Authors
Cited Authors
- Stone, KR; Mickey, DD; Wunderli, H; Mickey, GH; Paulson, DF
Published Date
- March 15, 1978
Published In
Volume / Issue
- 21 / 3
Start / End Page
- 274 - 281
PubMed ID
- 631930
International Standard Serial Number (ISSN)
- 0020-7136
Digital Object Identifier (DOI)
- 10.1002/ijc.2910210305
Language
- eng
Conference Location
- United States