Isolation of a human prostate carcinoma cell line (DU 145).

Journal Article (Journal Article)

A long-term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.

Full Text

Duke Authors

Cited Authors

  • Stone, KR; Mickey, DD; Wunderli, H; Mickey, GH; Paulson, DF

Published Date

  • March 15, 1978

Published In

Volume / Issue

  • 21 / 3

Start / End Page

  • 274 - 281

PubMed ID

  • 631930

International Standard Serial Number (ISSN)

  • 0020-7136

Digital Object Identifier (DOI)

  • 10.1002/ijc.2910210305


  • eng

Conference Location

  • United States