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Isolation of a human prostate carcinoma cell line (DU 145).

Publication ,  Journal Article
Stone, KR; Mickey, DD; Wunderli, H; Mickey, GH; Paulson, DF
Published in: Int J Cancer
March 15, 1978

A long-term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.

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Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

March 15, 1978

Volume

21

Issue

3

Start / End Page

274 / 281

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Male
  • Humans
  • Culture Techniques
  • Chromosome Aberrations
  • Cell Line
  • Brain Neoplasms
  • Brain
 

Citation

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Stone, K. R., Mickey, D. D., Wunderli, H., Mickey, G. H., & Paulson, D. F. (1978). Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer, 21(3), 274–281. https://doi.org/10.1002/ijc.2910210305
Stone, K. R., D. D. Mickey, H. Wunderli, G. H. Mickey, and D. F. Paulson. “Isolation of a human prostate carcinoma cell line (DU 145).Int J Cancer 21, no. 3 (March 15, 1978): 274–81. https://doi.org/10.1002/ijc.2910210305.
Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF. Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer. 1978 Mar 15;21(3):274–81.
Stone, K. R., et al. “Isolation of a human prostate carcinoma cell line (DU 145).Int J Cancer, vol. 21, no. 3, Mar. 1978, pp. 274–81. Pubmed, doi:10.1002/ijc.2910210305.
Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF. Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer. 1978 Mar 15;21(3):274–281.
Journal cover image

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

March 15, 1978

Volume

21

Issue

3

Start / End Page

274 / 281

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Male
  • Humans
  • Culture Techniques
  • Chromosome Aberrations
  • Cell Line
  • Brain Neoplasms
  • Brain