A bioluminescence method for the demonstration of regional glucose distribution in brain slices.

Published

Journal Article

Regional glucose distribution in brain slices was assessed by a bioluminescence technique. The reaction is based on light emission of luminiferous marine bacteria, Vibrio fischeri, induced by NADPH. Freeze-dried brain slices were covered by a solution which contained: (a) enzymes and substrates for glucose oxidation and NADPH formation and (b) an extract of Vibrio fischeri for the bioluminescence reaction. Glucose-induced bioluminescence was recorded on photographic film. Patterns of regional decrease in glucose concentration were demonstrated in cat brains after occlusion of the left middle cerebral artery. This decrease correlated well with a concomitant depletion of ATP and an increase in NADH-fluorescence.

Full Text

Cited Authors

  • Paschen, W; Niebuhr, I; Hossmann, KA

Published Date

  • February 1, 1981

Published In

Volume / Issue

  • 36 / 2

Start / End Page

  • 513 - 517

PubMed ID

  • 7463075

Pubmed Central ID

  • 7463075

Electronic International Standard Serial Number (EISSN)

  • 1471-4159

International Standard Serial Number (ISSN)

  • 0022-3042

Digital Object Identifier (DOI)

  • 10.1111/j.1471-4159.1981.tb01622.x

Language

  • eng