Changes in interferon-regulatory factor-1 mRNA levels after transient ischemia in rat brain.


Journal Article

To evaluate whether the interferon system in the brain is activated by a severe form of metabolic stress, and to elucidate the possible mechanism underlying this activation, changes in the interferon regulatory factor-1 (irf-1) mRNA levels were evaluated after transient cerebral ischemia, and after exposure of primary neuronal cells to experimental conditions resulting in a depletion of ER calcium stores. Following 30 min ischemia and 2 h recovery, irf-1 mRNA levels rose significantly and stayed high for up to 24 h of recovery. Irf-1 mRNA levels were also significantly increased in neurons in vitro after exposing cells to conditions resulting in ER calcium store depletion with or without a parallel increase in cytoplasmic calcium activity. It is concluded that transient cerebral ischemia induces activation of the interferon system and that disturbances of ER calcium homeostasis may play a role in this process.

Full Text

Cited Authors

  • Paschen, W; Gissel, C; Althausen, S; Doutheil, J

Published Date

  • October 1998

Published In

Volume / Issue

  • 9 / 14

Start / End Page

  • 3147 - 3151

PubMed ID

  • 9831442

Pubmed Central ID

  • 9831442

Electronic International Standard Serial Number (EISSN)

  • 1473-558X

International Standard Serial Number (ISSN)

  • 0959-4965

Digital Object Identifier (DOI)

  • 10.1097/00001756-199810050-00005


  • eng