A monitor for bud emergence in the yeast morphogenesis checkpoint.
Journal Article (Journal Article)
Cell cycle transitions are subject to regulation by both external signals and internal checkpoints that monitor satisfactory progression of key cell cycle events. In budding yeast, the morphogenesis checkpoint arrests the cell cycle in response to perturbations that affect the actin cytoskeleton and bud formation. Herein, we identify a step in this checkpoint pathway that seems to be directly responsive to bud emergence. Activation of the kinase Hsl1p is dependent upon its recruitment to a cortical domain organized by the septins, a family of conserved filament-forming proteins. Under conditions that delayed or blocked bud emergence, Hsl1p recruitment to the septin cortex still took place, but hyperphosphorylation of Hsl1p and recruitment of the Hsl1p-binding protein Hsl7p to the septin cortex only occurred after bud emergence. At this time, the septin cortex spread to form a collar between mother and bud, and Hsl1p and Hsl7p were restricted to the bud side of the septin collar. We discuss models for translating cellular geometry (in this case, the emergence of a bud) into biochemical signals regulating cell proliferation.
Full Text
Duke Authors
Cited Authors
- Theesfeld, CL; Zyla, TR; Bardes, EGS; Lew, DJ
Published Date
- August 2003
Published In
Volume / Issue
- 14 / 8
Start / End Page
- 3280 - 3291
PubMed ID
- 12925763
Pubmed Central ID
- PMC181567
International Standard Serial Number (ISSN)
- 1059-1524
Digital Object Identifier (DOI)
- 10.1091/mbc.e03-03-0154
Language
- eng
Conference Location
- United States