Overlapping elements in the guanylate-binding protein gene promoter mediate transcriptional induction by alpha and gamma interferons.

Journal Article

The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is transcriptionally induced in cells exposed to interferon of either type I (alpha interferon [IFN-alpha] or type II (IFN-gamma). The promoter of the GBP gene was cloned and found to contain an IFN-alpha-stimulated response element, which mediated the response of the GBP gene to IFN-alpha. On the basis of transfection experiments with recombinant plasmids, two different elements were delineated. Both were required to obtain the maximal response of the GBP gene to IFN-gamma: the IFN-alpha-stimulated response element and an overlapping element termed the IFN-gamma activation site. Different proteins that act on each element were investigated, and their possible involvement in IFN-gamma-induced transcriptional regulation is discussed.

Full Text

Duke Authors

Cited Authors

  • Lew, DJ; Decker, T; Strehlow, I; Darnell, JE

Published Date

  • January 1991

Published In

Volume / Issue

  • 11 / 1

Start / End Page

  • 182 - 191

PubMed ID

  • 1898761

International Standard Serial Number (ISSN)

  • 0270-7306

Language

  • eng

Conference Location

  • United States