Stress-specific activation mechanisms for the "cell integrity" MAPK pathway.


Journal Article

Many environmental stresses trigger cellular responses by activating mitogen-activated protein kinase (MAPK) pathways. Once activated, these highly conserved protein kinase cascades can elicit cellular responses such as transcriptional activation of response genes, cytoskeletal rearrangement, and cell cycle arrest. The mechanism of pathway activation by environmental stresses is in most cases unknown. We have analyzed the activation of the budding yeast "cell integrity" MAPK pathway by heat shock, hypoosmotic shock, and actin perturbation, and we report that different stresses regulate this pathway at different steps. In no case can MAPK activation be explained by the prevailing view that stresses simply induce GTP loading of the Rho1p GTPase at the "top" of the pathway. Instead, our findings suggest that the stresses can modulate at least three distinct kinases acting between Rho1p and the MAPK. These findings suggest that stresses provide "lateral" inputs into this regulatory pathway, rather than operating in a linear "top-down" manner.

Full Text

Duke Authors

Cited Authors

  • Harrison, JC; Zyla, TR; Bardes, ESG; Lew, DJ

Published Date

  • January 23, 2004

Published In

Volume / Issue

  • 279 / 4

Start / End Page

  • 2616 - 2622

PubMed ID

  • 14610085

Pubmed Central ID

  • 14610085

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M306110200


  • eng

Conference Location

  • United States