Advances in the electroanalytical technology of NO detection make it possible to detect the release of robust concentrations of NO from living systems under pathological or pharmacological conditions. However, technical improvements should enable the construction of research instruments with one or two orders of magnitude improvement in both detection limit and temporal resolution. Such instruments would be capable of revealing physiological NO production and could help quantify the correlations between NO levels and health or disease, ultimately leading to important applications in biomedical research and clinical medicine.