Electrochemical detection of nitric oxide in biological fluids.
Publication
, Journal Article
Allen, BW; Liu, J; Piantadosi, CA
Published in: Methods Enzymol
2005
The challenges that must be overcome in order to detect nitric oxide (NO) in biological fluids include its low physiological concentration (1-nM) and its short half-life (a few seconds or less). Electrochemistry is capable of making such measurements, if certain principles, both biological and electrochemical, are kept in mind. We discuss these principles and demonstrate an example of practical measurement by detecting NO release in a drop of blood suspended within the reference electrode of an electrochemical cell. We elicit the NO release by decreasing the oxygen concentration in the gaseous atmosphere surrounding the drop.
Duke Scholars
Published In
Methods Enzymol
DOI
ISSN
0076-6879
Publication Date
2005
Volume
396
Start / End Page
68 / 77
Location
United States
Related Subject Headings
- Sensitivity and Specificity
- Platinum
- Nitric Oxide
- Microscopy, Electron, Scanning
- Electrodes
- Electrochemistry
- Biochemistry & Molecular Biology
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Allen, B. W., Liu, J., & Piantadosi, C. A. (2005). Electrochemical detection of nitric oxide in biological fluids. Methods Enzymol, 396, 68–77. https://doi.org/10.1016/S0076-6879(05)96007-2
Allen, Barry W., Jie Liu, and Claude A. Piantadosi. “Electrochemical detection of nitric oxide in biological fluids.” Methods Enzymol 396 (2005): 68–77. https://doi.org/10.1016/S0076-6879(05)96007-2.
Allen BW, Liu J, Piantadosi CA. Electrochemical detection of nitric oxide in biological fluids. Methods Enzymol. 2005;396:68–77.
Allen, Barry W., et al. “Electrochemical detection of nitric oxide in biological fluids.” Methods Enzymol, vol. 396, 2005, pp. 68–77. Pubmed, doi:10.1016/S0076-6879(05)96007-2.
Allen BW, Liu J, Piantadosi CA. Electrochemical detection of nitric oxide in biological fluids. Methods Enzymol. 2005;396:68–77.
Published In
Methods Enzymol
DOI
ISSN
0076-6879
Publication Date
2005
Volume
396
Start / End Page
68 / 77
Location
United States
Related Subject Headings
- Sensitivity and Specificity
- Platinum
- Nitric Oxide
- Microscopy, Electron, Scanning
- Electrodes
- Electrochemistry
- Biochemistry & Molecular Biology
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology