Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.

Published

Journal Article

beta-Catenin is a multifunctional protein that is known to participate in two well defined cellular processes, cell-cell adhesion and Wnt-stimulated transcriptional activation. Here we report that beta-catenin participates in a third cellular process, the establishment of a bipolar mitotic spindle. During mitosis, beta-catenin relocalizes to mitotic spindle poles and to the midbody. Furthermore, biochemical fractionation demonstrates the presence of beta-catenin in purified centrosome preparations. Reduction of cellular beta-catenin by RNA interference leads to the failure of centrosomes to fully separate, resulting in a marked increase in the frequency of monoastral mitotic spindles. Our results define a new and important function for beta-catenin in mitosis and demonstrate that beta-catenin is involved in vital biological processes beyond cell adhesion and Wnt signaling.

Full Text

Duke Authors

Cited Authors

  • Kaplan, DD; Meigs, TE; Kelly, P; Casey, PJ

Published Date

  • March 19, 2004

Published In

Volume / Issue

  • 279 / 12

Start / End Page

  • 10829 - 10832

PubMed ID

  • 14744872

Pubmed Central ID

  • 14744872

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.C400035200

Language

  • eng

Conference Location

  • United States