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Association of misexpression with sterility in hybrids of Drosophila simulansand D. mauritiana.

Publication ,  Journal Article
Michalak, P; Noor, MAF
Published in: Journal of molecular evolution
August 2004

Recent studies have identified genes associated with hybrid sterility and other hybrid dysfunctions, but the consequences of introgressions of these "speciation genes" are often poorly understood. Previously, we identified a panel of genes that are underexpressed in sterile male hybrids of Drosophila simulans and D. mauritiana relative to pure species. Here, we build on this reverse-genetics approach to demonstrate that the underexpression of at least five of these genes in hybrids is associated with hybrid sterility and that these five genes are coordinately regulated. We map one upstream regulator of these genes to a region previously shown to harbor one or more factors causing hybrid sterility. Finally, we show that the genes underexpressed in hybrids are often highly conserved, as might be predicted for downstream targets of the genetic changes that cause hybrid sterility. This approach integrates forward genetics with reverse genetics to show a proximate consequence of the introgression of particular hybrid sterility-conferring regions between species: underexpression of genes necessary for normal spermatogenesis.

Duke Scholars

Published In

Journal of molecular evolution

DOI

EISSN

1432-1432

ISSN

0022-2844

Publication Date

August 2004

Volume

59

Issue

2

Start / End Page

277 / 282

Related Subject Headings

  • Spermatogenesis
  • Sequence Analysis, DNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Reproduction
  • Molecular Sequence Data
  • Male
  • Hybridization, Genetic
  • Genotype
  • Genetic Variation
  • Genes, Insect
 

Citation

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Michalak, P., & Noor, M. A. F. (2004). Association of misexpression with sterility in hybrids of Drosophila simulansand D. mauritiana. Journal of Molecular Evolution, 59(2), 277–282. https://doi.org/10.1007/s00239-004-2622-y
Michalak, Pawel, and Mohamed A. F. Noor. “Association of misexpression with sterility in hybrids of Drosophila simulansand D. mauritiana.Journal of Molecular Evolution 59, no. 2 (August 2004): 277–82. https://doi.org/10.1007/s00239-004-2622-y.
Michalak P, Noor MAF. Association of misexpression with sterility in hybrids of Drosophila simulansand D. mauritiana. Journal of molecular evolution. 2004 Aug;59(2):277–82.
Michalak, Pawel, and Mohamed A. F. Noor. “Association of misexpression with sterility in hybrids of Drosophila simulansand D. mauritiana.Journal of Molecular Evolution, vol. 59, no. 2, Aug. 2004, pp. 277–82. Epmc, doi:10.1007/s00239-004-2622-y.
Michalak P, Noor MAF. Association of misexpression with sterility in hybrids of Drosophila simulansand D. mauritiana. Journal of molecular evolution. 2004 Aug;59(2):277–282.
Journal cover image

Published In

Journal of molecular evolution

DOI

EISSN

1432-1432

ISSN

0022-2844

Publication Date

August 2004

Volume

59

Issue

2

Start / End Page

277 / 282

Related Subject Headings

  • Spermatogenesis
  • Sequence Analysis, DNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Reproduction
  • Molecular Sequence Data
  • Male
  • Hybridization, Genetic
  • Genotype
  • Genetic Variation
  • Genes, Insect