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Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis.

Publication ,  Journal Article
Noor, MAF; Michalak, P; Donze, D
Published in: Genetics
December 2003

Characterizing genes that are misregulated in hybrids may elucidate the genetic basis of hybrid sterility or other hybrid dysfunctions that contribute to speciation. Previously, a small segment of a male-predominant transcript that is underexpressed in adult male hybrids of Drosophila pseudoobscura and D. persimilis relative to pure species was identified in a differential display screen. Here, we obtained the full sequence of this 1330-bp transcript and determined that it is an antisense message with high sequence similarity to the D. melanogaster TRAP100 gene, part of the Mediator protein complex that regulates transcriptional initiation during development. Both the sense and the antisense messages are transcribed in D. pseudoobscura, but only the sense message (TRAP100) is transcribed in D. melanogaster complex species. Unlike the antisense message, the sense message is transcribed similarly in D. pseudoobscura males and females and in hybrids of D. pseudoobscura and D. persimilis. The high sequence similarity between distantly related species suggests that the sense message is functionally constrained within the genus. We speculate that the antisense transcript may have evolved a role in male-specific post-transcriptional regulation of TRAP100 in the D. pseudoobscura lineage and that its underexpression in sterile hybrid males may cause an overproduction of TRAP100 protein, possibly yielding deleterious effects.

Duke Scholars

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

December 2003

Volume

165

Issue

4

Start / End Page

1823 / 1830

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • RNA, Antisense
  • Molecular Sequence Data
  • Male
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Female
 

Citation

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Noor, M. A. F., Michalak, P., & Donze, D. (2003). Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis. Genetics, 165(4), 1823–1830. https://doi.org/10.1093/genetics/165.4.1823
Noor, Mohamed A. F., Pawel Michalak, and David Donze. “Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis.Genetics 165, no. 4 (December 2003): 1823–30. https://doi.org/10.1093/genetics/165.4.1823.
Noor, Mohamed A. F., et al. “Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis.Genetics, vol. 165, no. 4, Dec. 2003, pp. 1823–30. Epmc, doi:10.1093/genetics/165.4.1823.

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

December 2003

Volume

165

Issue

4

Start / End Page

1823 / 1830

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • RNA, Antisense
  • Molecular Sequence Data
  • Male
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Female