A human-human hybridoma secreting anti-HLA class II antibody.


Journal Article

In this report, we describe the production and characterization of the first human-human hybridoma secreting antibody to HLA Class II determinants. The hybridoma (GMEC101), which has been stable in tissue culture for greater than 20 mo, secretes 10 to 50 micrograms/ml of IgM-kappa antibody. This antibody binds to a wide range of human cell lines, but not to the HLA-A,B,C, and DR-negative K562 cell line. Functionally, GMEC101 strongly inhibits a unidirectional mixed lymphocyte reaction (MLR) at the level of the stimulator cell. Neither the cellular ELISA binding nor the MLR inhibition is lost after a triple platelet absorption (which removes Class I but not Class II activity). Because the binding and MLR blocking show no correlation with the known DR or DQ specificities, we suggest that GMEC101 may be detecting a novel HLA Class II determinant.

Full Text

Cited Authors

  • Effros, RB; Hulette, CM; Ettenger, R; Dillard, LC; Zeller, E; Duong, R; Walford, RL

Published Date

  • September 1, 1986

Published In

Volume / Issue

  • 137 / 5

Start / End Page

  • 1599 - 1603

PubMed ID

  • 2943797

Pubmed Central ID

  • 2943797

International Standard Serial Number (ISSN)

  • 0022-1767


  • eng

Conference Location

  • United States