ELISA-quantitation of phosphorylated tau protein in the Alzheimer's disease brain.

Journal Article

A reliable, sensitive and specific sandwich ELISA for the quantitation of paired helical filament (PHF) tau in human brain was developed using well-defined monoclonal antibodies. We examined rapid-autopsy-derived brain tissue from 21 neuropathologically confirmed Alzheimer's disease (AD) patients and 14 nondemented controls, matched for age, sex and postmortem delay times. We demonstrated significant elevations of phosphorylated tau levels in the frontal and parietal cortex as well as in the hippocampus of AD patients as compared to the nondemented controls. No difference was observed in the cerebellum. Phosphorylated tau levels measured by ELISA were significantly correlated with the presence or absence of neurofibrillary tangles.

Full Text

Duke Authors

Cited Authors

  • Herrmann, M; Golombowski, S; Kräuchi, K; Frey, P; Mourton-Gilles, C; Hulette, C; Rosenberg, C; Müller-Spahn, F; Hock, C

Published Date

  • 1999

Published In

Volume / Issue

  • 42 / 4

Start / End Page

  • 205 - 210

PubMed ID

  • 10567816

International Standard Serial Number (ISSN)

  • 0014-3022

Digital Object Identifier (DOI)

  • 10.1159/000008108

Language

  • eng

Conference Location

  • Switzerland