In-vitro exposure of guinea pig main bronchi to 2.5 ppm of nitrogen dioxide does not alter airway smooth muscle response.

Published

Journal Article

In order to investigate whether the oxidant airborne pollutant nitrogen dioxide (NO2) affects airway smooth muscle responsiveness, the contractile response of guinea pig main bronchi after in vitro exposure to 2.5 ppm of nitrogen dioxide was studied. Main bronchi were cannulated and exposed for 2 or 4 h to a constant flow of either NO2 or air. After exposure, bronchial rings were obtained and placed in a 37 degrees C jacketed organ bath filled with Krebs-Henseleit solution. Concentration-response curves were performed for acetylcholine (10(-9)-10(-3) M), substance P (10(-9)-10(-4) M), and neurokinin A (10(-10)-10(-5) M), and voltage-response curves (12-28 V) were performed for electrical field stimulation. There was no significant difference in either the smooth muscle maximal contractile response, or sensitivity between the bronchi exposed to NO2 and those exposed to air. We conclude that in vitro exposure to 2.5 ppm of NO2 does not alter airway smooth muscle responsiveness in guinea pigs.

Full Text

Cited Authors

  • Chitano, P; Lucchini, RE; Coser, E; Papi, A; Saetta, M; Maestrelli, P; Ciaccia, A; Fabbri, LM; Mapp, CE

Published Date

  • May 1995

Published In

Volume / Issue

  • 89 / 5

Start / End Page

  • 323 - 328

PubMed ID

  • 7543687

Pubmed Central ID

  • 7543687

Electronic International Standard Serial Number (EISSN)

  • 1532-3064

International Standard Serial Number (ISSN)

  • 0954-6111

Digital Object Identifier (DOI)

  • 10.1016/0954-6111(95)90002-0

Language

  • eng