Phylogenetic analyses suggest reverse splicing spread of group I introns in fungal ribosomal DNA.

Journal Article (Journal Article)

Background

Group I introns have spread into over 90 different sites in nuclear ribosomal DNA (rDNA) with greater than 1700 introns reported in these genes. These ribozymes generally spread through endonuclease-mediated intron homing. Another putative pathway is reverse splicing whereby a free group I intron inserts into a homologous or heterologous RNA through complementary base-pairing between the intron and exon RNA. Reverse-transcription of the RNA followed by general recombination results in intron spread. Here we used phylogenetics to test for reverse splicing spread in a taxonomically broadly sampled data set of fungal group I introns including 9 putatively ancient group I introns in the rDNA of the yeast-like symbiont Symbiotaphrina buchneri.

Results

Our analyses reveal a complex evolutionary history of the fungal introns with many cases of vertical inheritance (putatively for the 9 introns in S. buchneri) and intron lateral transfer. There are several examples in which introns, many of which are still present in S. buchneri, may have spread through reverse splicing into heterologous rDNA sites. If the S. buchneri introns are ancient as we postulate, then group I intron loss was widespread in fungal rDNA evolution.

Conclusion

On the basis of these results, we suggest that the extensive distribution of fungal group I introns is at least partially explained by the reverse splicing movement of existing introns into ectopic rDNA sites.

Full Text

Duke Authors

Cited Authors

  • Bhattacharya, D; Reeb, V; Simon, DM; Lutzoni, F

Published Date

  • November 21, 2005

Published In

Volume / Issue

  • 5 /

Start / End Page

  • 68 -

PubMed ID

  • 16300679

Pubmed Central ID

  • PMC1299323

Electronic International Standard Serial Number (EISSN)

  • 1471-2148

International Standard Serial Number (ISSN)

  • 1471-2148

Digital Object Identifier (DOI)

  • 10.1186/1471-2148-5-68

Language

  • eng