Creation and properties of histocompatibility antigen-cell conjugates. I. Preparation and immunological properties of rat histocompatibility antigens.

Published

Journal Article

A necessary step in the induction of unresponsiveness to transplantation antigens is the preparation and presentation of tissue antigens in a suitable form. A method is presented for obtaining rat histocompatibility antigen in a soluble, "heptenic" form suitable for coupling to a tolerogencie carrier. Lewis and ACI spleen cell membranes are solubilized with deoxycholate and purified by Lens culinaris affinity chromatography. The ultracentrifuged, solubilized preparation of rat histocompatibility (HC) antigen is unable to initiate immunity, but is able to react specifically with the product of an already established immune response. The HC antigen preparation is neither immunogenic nor tolerogenic alone. However, an antibody response to allogeneic cells can be prevented when these rat HC preparations are chemically coupled to tolerogenic carrier as described in the accompanying paper.

Full Text

Duke Authors

Cited Authors

  • Bollinger, RR; Scott, DW

Published Date

  • June 1980

Published In

Volume / Issue

  • 29 / 6

Start / End Page

  • 444 - 448

PubMed ID

  • 6157235

Pubmed Central ID

  • 6157235

International Standard Serial Number (ISSN)

  • 0041-1337

Digital Object Identifier (DOI)

  • 10.1097/00007890-198006000-00003

Language

  • eng

Conference Location

  • United States