Suppression of hepatic allograft rejection in the rat by mitomycin C-treated donor splenocytes: analysis of the immune status.

Published

Journal Article

A single intraperitoneal injection of 3 x 10(6) donor splenocytes treated with mitomycin c (MMC) seven days before hepatic transplantation prolongs survival of hepatic allografts in the ACI (RT1a) to LEW (RT1l) rat combination. This effect is donor specific. An intravenous injection of the same dose of splenocytes treated with MMC seven days before transplantation also tends to prolong hepatic allograft survival. Furthermore, lymphocytotoxic antibody can be detected in rats 30 days after transplantation. Adoptive transfer of 5 x 10(7) splenocytes taken from long-term surviving hepatic allograft recipients pretreated with MMC-treated donor ACI splenocytes into irradiated (750 rads) LEW rats prolongs the survival of donor-type skin grafts, whereas third-party strain (BN) grafts are rejected. Similarly, prolonged survival of ACI cardiac allografts in irradiated (450 rads) LEW recipients is achieved following the transfer of spleen cells taken from longterm surviving hepatic allograft recipients pretreated with MMC-treated donor ACI splenocytes, whereas third-party (BN) cardiac allografts show rejection. These findings suggest the presence of donor-specific suppressor cells and indicate that a single injection of donor splenocytes treated with MMC to the recipient seven days before transplantation can induce specific suppression of rejection in a rat hepatic allograft model.

Full Text

Duke Authors

Cited Authors

  • Yamaguchi, Y; Mori, K; Bollinger, RR

Published Date

  • June 1, 1990

Published In

Volume / Issue

  • 32 / 2

Start / End Page

  • 59 - 66

PubMed ID

  • 1967040

Pubmed Central ID

  • 1967040

International Standard Serial Number (ISSN)

  • 0141-2760

Language

  • eng

Conference Location

  • Scotland