Linkage analysis in familial amyotrophic lateral sclerosis.


Journal Article

Familial amyotrophic lateral sclerosis (FALS) constitutes 5 to 10% of cases of ALS and, in most families, its inheritance is consistent with an autosomal dominant trait with age-dependent penetrance. The biochemical abnormality underlying the disorder is unknown. We analyzed DNA from 131 members of 6 multigenerational ALS families, which included 13 affected members, for genetic linkage to 39 expressed and DNA markers, using the techniques of 2-point linkage analysis, multilocus linkage analysis, and exclusion mapping. We identified FALS families with structures suitable for linkage, by computer simulation techniques. A DNA bank established to provide optimum use of available FALS families provided DNA from immortalized lymphoblast cell lines and frozen postmortem tissue. We could not link FALS to any of the markers studied, but excluded chromosome regions unlikely to be a locus of the FALS gene. With the help of this exclusion data, we will concentrate on regions of the human genome that remain unexcluded.

Full Text

Duke Authors

Cited Authors

  • Siddique, T; Pericak-Vance, MA; Brooks, BR; Roos, RP; Hung, WY; Antel, JP; Munsat, TL; Phillips, K; Warner, K; Speer, M

Published Date

  • July 1, 1989

Published In

Volume / Issue

  • 39 / 7

Start / End Page

  • 919 - 925

PubMed ID

  • 2739919

Pubmed Central ID

  • 2739919

International Standard Serial Number (ISSN)

  • 0028-3878

Digital Object Identifier (DOI)

  • 10.1212/wnl.39.7.919


  • eng

Conference Location

  • United States