Genetic structure of typical and atypical populations of Candida albicans from Africa.

Journal Article (Journal Article)

Atypical isolates of the pathogenic yeast Candida albicans have been reported with increasing frequency. To investigate the origin of a set of atypical isolates and their relationship to typical isolates, we employed a combination of molecular phylogenetic and population genetic analyses using rDNA sequencing, PCR fingerprinting, and analysis of co-dominant DNA nucleotide polymorphisms to characterize the population structure of one typical and two atypical populations of C. albicans from Angola and Madagascar. The extent of clonality and recombination was assessed in each population. The analyses revealed that the structure of all three populations of C. albicans was predominantly clonal but, as in previous studies, there was also evidence for recombination. Allele frequencies differed significantly between the typical and the atypical populations, suggesting very low levels of gene flow between them. However, allele frequencies were quite similar in the two atypical C. albicans populations, suggesting that they are closely related. Phylogenetic analysis of partial sequences encoding the nuclear 26S rDNA demonstrated that all three populations belong to a single monophyletic group, which includes the type strain of C. albicans.

Full Text

Duke Authors

Cited Authors

  • Forche, A; Schönian, G; Gräser, Y; Vilgalys, R; Mitchell, TG

Published Date

  • November 1999

Published In

Volume / Issue

  • 28 / 2

Start / End Page

  • 107 - 125

PubMed ID

  • 10587473

International Standard Serial Number (ISSN)

  • 1087-1845

Digital Object Identifier (DOI)

  • 10.1006/fgbi.1999.1164


  • eng

Conference Location

  • United States