Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxoplasma gondii.

Journal Article (Journal Article)

Two new methods for the detection of antibodies to Toxoplasma gondii, an enzyme-linked immunosorbent assay and a quantitative immunofluorescence assay, were evaluated and compared with the conventional indirect fluorescent-antibody slide test. Each of 100 human sera was assayed twice by the three procedures. Both the enzyme-linked immunosorbent assay and the quantitative immunofluorescence assay correlated well with serologically positive (indirect fluorescent-antibody titer greater than or equal to 1:32) and negative sera. The enzyme-linked immunosorbent assay was more specific, but less sensitive, than the quantitative immunofluorescence assay. However, the quantitative immunofluorescence assay was more reproducible and more rapid than the enzyme-linked immunosorbent assay.

Full Text

Duke Authors

Cited Authors

  • Violand, SA; Mitchell, TG; Kleeman, KT

Published Date

  • August 1, 1982

Published In

Volume / Issue

  • 16 / 2

Start / End Page

  • 341 - 344

PubMed ID

  • 6749896

Pubmed Central ID

  • PMC272357

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.16.2.341-344.1982


  • eng

Conference Location

  • United States