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Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis.

Publication ,  Journal Article
Hill, HR; Hogan, NA; Mitchell, TG; Quie, PG
Published in: J Lab Clin Med
October 1975

An in vitro method for the assessment of neutrophil granulocyte chemotaxis employing a cytocentrifuge has been evaluated. The cytocentrifuge is used to directly sediment leukocytes on a Millipore filter, and thus (1) eliminates errors due to abnormal or unequal settling of the cells and (2) eliminates the need for serum, plasma, or albumin on the cell side of the chemotactic chamber to obtain optimal chemotaxis. In addition, other advantages of the method are that it requires smaller volumes of blood, less handling of the cells, and less time is required for visual counting of the neutrophils. The method has been utilized to assess chemotactic activity of neutrophils from 80 control subjects and over 100 patients with various diseases and to study the effects of various pharmacologic agents on neutrophil chemotactic responsiveness.

Duke Scholars

Published In

J Lab Clin Med

ISSN

0022-2143

Publication Date

October 1975

Volume

86

Issue

4

Start / End Page

703 / 710

Location

United States

Related Subject Headings

  • Phagocytosis
  • Neutrophils
  • Leukocyte Count
  • In Vitro Techniques
  • Humans
  • Granulocytes
  • General Clinical Medicine
  • Evaluation Studies as Topic
  • Chemotaxis
  • Centrifugation
 

Citation

APA
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ICMJE
MLA
NLM
Hill, H. R., Hogan, N. A., Mitchell, T. G., & Quie, P. G. (1975). Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis. J Lab Clin Med, 86(4), 703–710.
Hill, H. R., N. A. Hogan, T. G. Mitchell, and P. G. Quie. “Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis.J Lab Clin Med 86, no. 4 (October 1975): 703–10.
Hill HR, Hogan NA, Mitchell TG, Quie PG. Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis. J Lab Clin Med. 1975 Oct;86(4):703–10.
Hill, H. R., et al. “Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis.J Lab Clin Med, vol. 86, no. 4, Oct. 1975, pp. 703–10.
Hill HR, Hogan NA, Mitchell TG, Quie PG. Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis. J Lab Clin Med. 1975 Oct;86(4):703–710.
Journal cover image

Published In

J Lab Clin Med

ISSN

0022-2143

Publication Date

October 1975

Volume

86

Issue

4

Start / End Page

703 / 710

Location

United States

Related Subject Headings

  • Phagocytosis
  • Neutrophils
  • Leukocyte Count
  • In Vitro Techniques
  • Humans
  • Granulocytes
  • General Clinical Medicine
  • Evaluation Studies as Topic
  • Chemotaxis
  • Centrifugation