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Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera.

Publication ,  Journal Article
Manning-Zweerink, M; Maloney, CS; Mitchell, TG; Weston, H
Published in: J Clin Microbiol
January 1986

We tested 10 patient sera for the presence of immunoglobulin G (IgG) antibodies to Candida albicans and for C. albicans antigens by immunoblot analysis (i.e., electrotransfer blot radioimmunoassay) (G. E. Smith and M. D. Summers, J. Virol. 39:125-137, 1981). We evaluated sera from two patients at risk for candidiasis, five patients with systemic candidiasis documented by culture, and two patients who had experienced transient candidemia. Both the specificity and the relative amount of IgG antibodies to C. albicans in each serum sample were readily visualized by this technique, as was the absence of antibody from serum of neonatal and immunocompromised patients. No antibody species appeared to be uniquely associated with candidiasis patients (i.e., each antibody species present in the candidiasis patient was also present in sera of normal individuals or "at-risk" patients). IgG from rabbits immunized with whole cells or with a cytoplasmic fraction of C. albicans was used to detect C. albicans antigens in patient sera. Although several antigens were detected in the sera from patients with candidiasis, the same antigens were also detected in sera from patients at risk and in normal human serum. No antigens were detected in human serum when preimmune rabbit sera were used. These results suggest that the antigens detected by the rabbit antisera were human serum proteins that cross-reacted with C. albicans antigens. These findings may have important implications in studies of both the pathobiology of C. albicans and the serodiagnosis of candidiasis.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

January 1986

Volume

23

Issue

1

Start / End Page

46 / 52

Location

United States

Related Subject Headings

  • Radioimmunoassay
  • Microbiology
  • Immunoglobulin G
  • Immune Sera
  • Humans
  • Epitopes
  • Electrophoresis, Polyacrylamide Gel
  • Cross Reactions
  • Candidiasis
  • Candida albicans
 

Citation

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Manning-Zweerink, M., Maloney, C. S., Mitchell, T. G., & Weston, H. (1986). Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera. J Clin Microbiol, 23(1), 46–52. https://doi.org/10.1128/jcm.23.1.46-52.1986
Manning-Zweerink, M., C. S. Maloney, T. G. Mitchell, and H. Weston. “Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera.J Clin Microbiol 23, no. 1 (January 1986): 46–52. https://doi.org/10.1128/jcm.23.1.46-52.1986.
Manning-Zweerink M, Maloney CS, Mitchell TG, Weston H. Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera. J Clin Microbiol. 1986 Jan;23(1):46–52.
Manning-Zweerink, M., et al. “Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera.J Clin Microbiol, vol. 23, no. 1, Jan. 1986, pp. 46–52. Pubmed, doi:10.1128/jcm.23.1.46-52.1986.
Manning-Zweerink M, Maloney CS, Mitchell TG, Weston H. Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera. J Clin Microbiol. 1986 Jan;23(1):46–52.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

January 1986

Volume

23

Issue

1

Start / End Page

46 / 52

Location

United States

Related Subject Headings

  • Radioimmunoassay
  • Microbiology
  • Immunoglobulin G
  • Immune Sera
  • Humans
  • Epitopes
  • Electrophoresis, Polyacrylamide Gel
  • Cross Reactions
  • Candidiasis
  • Candida albicans