Isolation of Cryptococcus gattii and Cryptococcus neoformans var. grubii from the flowers and bark of Eucalyptus trees in India.

Published

Journal Article

The association of Cryptococcus gattii with Eucalyptus trees has been well established. Here we report the isolation of both C. gattii and Cryptococcus neoformans var. grubii from the flowers and bark of Eucalyptus trees in India. We investigated a total of 233 samples of Eucalyptus trees: 120 flowers, 81 fragments of bark, and 32 leaves. C. gattii was isolated from two samples of flowers of Eucalyptus terreticornis. C. neoformans var. grubii was recovered twice from the bark of Eucalyptus camaldulensis, initially from one of three samples, and again 2 months later, from one of four samples collected beneath the canopy of the tree. The primary isolation medium was Nigerseed agar, and brown colonies were presumptively identified as C. gattii or C. neoformans. The species identification was confirmed by morphological and biochemical characteristics. Using the Crypto-Check kit (Iatron, Tokyo, Japan), the first two isolates were identified as serotype B (C. gattii) and the other two were serotype A (C. neoformans var. grubii). PCR analysis of the isolates of C. neoformans var. grubii revealed that they possessed the MATalpha mating type allele. Molecular typing by amplified fragment length polymorphism markers indicated that both isolates of C. neoformans var. grubii possessed the same genotype. This study demonstrates that C. neoformans var. grubii, as well as C. gattii, may be associated with Eucalyptus trees.

Full Text

Duke Authors

Cited Authors

  • Gugnani, HC; Mitchell, TG; Litvintseva, AP; Lengeler, KB; Heitman, J; Kumar, A; Basu, S; Paliwal-Joshi, A

Published Date

  • September 2005

Published In

Volume / Issue

  • 43 / 6

Start / End Page

  • 565 - 569

PubMed ID

  • 16323312

Pubmed Central ID

  • 16323312

International Standard Serial Number (ISSN)

  • 1369-3786

Digital Object Identifier (DOI)

  • 10.1080/13693780500160785

Language

  • eng

Conference Location

  • England