Skip to main content

Telomerase mRNA-transfected dendritic cells stimulate antigen-specific CD8+ and CD4+ T cell responses in patients with metastatic prostate cancer.

Publication ,  Journal Article
Su, Z; Dannull, J; Yang, BK; Dahm, P; Coleman, D; Yancey, D; Sichi, S; Niedzwiecki, D; Boczkowski, D; Gilboa, E; Vieweg, J
Published in: J Immunol
March 15, 2005

Telomerase reverse transcriptase (hTERT) represents an attractive target for cancer immunotherapy because hTERT is reactivated in most human tumors. A clinical trial was initiated in which hTERT mRNA-transfected dendritic cells (DC) were administered to 20 patients with metastatic prostate cancer. Nine of these subjects received DC transfected with mRNA encoding a chimeric lysosome-associated membrane protein-1 (LAMP) hTERT protein, allowing for concomitant induction of hTERT-specific CD8+ and CD4+ T cell responses. Treatment was well tolerated. Intense infiltrates of hTERT-specific T cells were noted at intradermal injection sites after repeated vaccination. In 19 of 20 subjects, expansion of hTERT-specific CD8+ T cells was measured in the peripheral blood of study subjects, with 0.9-1.8% of CD8+ T cells exhibiting Ag specificity. Patients immunized with the chimeric LAMP hTERT vaccine developed significantly higher frequencies of hTERT-specific CD4+ T cells than subjects receiving DC transfected with the unmodified hTERT template. Moreover, CTL-mediated killing of hTERT targets was enhanced in the LAMP hTERT group, suggesting that an improved CD4+ response could augment a CTL response. Vaccination was further associated with a reduction of prostate-specific Ag velocity and molecular clearance of circulating micrometastases. Our findings provide a rationale for further development of hTERT-transfected DC vaccines in the treatment of prostate and other cancers.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

March 15, 2005

Volume

174

Issue

6

Start / End Page

3798 / 3807

Location

United States

Related Subject Headings

  • Transfection
  • Telomerase
  • Safety
  • RNA, Messenger
  • Prostatic Neoplasms
  • Middle Aged
  • Male
  • Immunotherapy
  • Immunology
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Su, Z., Dannull, J., Yang, B. K., Dahm, P., Coleman, D., Yancey, D., … Vieweg, J. (2005). Telomerase mRNA-transfected dendritic cells stimulate antigen-specific CD8+ and CD4+ T cell responses in patients with metastatic prostate cancer. J Immunol, 174(6), 3798–3807. https://doi.org/10.4049/jimmunol.174.6.3798
Su, Zhen, Jens Dannull, Benjamin K. Yang, Philipp Dahm, Doris Coleman, Donna Yancey, Sylvia Sichi, et al. “Telomerase mRNA-transfected dendritic cells stimulate antigen-specific CD8+ and CD4+ T cell responses in patients with metastatic prostate cancer.J Immunol 174, no. 6 (March 15, 2005): 3798–3807. https://doi.org/10.4049/jimmunol.174.6.3798.
Su Z, Dannull J, Yang BK, Dahm P, Coleman D, Yancey D, et al. Telomerase mRNA-transfected dendritic cells stimulate antigen-specific CD8+ and CD4+ T cell responses in patients with metastatic prostate cancer. J Immunol. 2005 Mar 15;174(6):3798–807.
Su, Zhen, et al. “Telomerase mRNA-transfected dendritic cells stimulate antigen-specific CD8+ and CD4+ T cell responses in patients with metastatic prostate cancer.J Immunol, vol. 174, no. 6, Mar. 2005, pp. 3798–807. Pubmed, doi:10.4049/jimmunol.174.6.3798.
Su Z, Dannull J, Yang BK, Dahm P, Coleman D, Yancey D, Sichi S, Niedzwiecki D, Boczkowski D, Gilboa E, Vieweg J. Telomerase mRNA-transfected dendritic cells stimulate antigen-specific CD8+ and CD4+ T cell responses in patients with metastatic prostate cancer. J Immunol. 2005 Mar 15;174(6):3798–3807.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

March 15, 2005

Volume

174

Issue

6

Start / End Page

3798 / 3807

Location

United States

Related Subject Headings

  • Transfection
  • Telomerase
  • Safety
  • RNA, Messenger
  • Prostatic Neoplasms
  • Middle Aged
  • Male
  • Immunotherapy
  • Immunology
  • Humans