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Intraportal injection of monoclonal antibody in nude mice bearing hepatic metastases.

Publication ,  Journal Article
Rivoire, ML; Yoshida, K; Voiglio, EJ; Divgi, CR; Niedzwiecki, D; Chapman, D; Cohen, AM; Sigurdson, ER
Published in: J Surg Oncol
October 1993

Using a model for hepatic human colorectal carcinoma metastases in athymic mice, we compared the selective [intraportal (ip)] and systemic [intravenous (iv)] injection of radiolabeled monoclonal antibody (mAb) strongly reactive against the cell line. Percent injected dose of radiolabeled antibody per gram (%id/g) of tumor or normal tissues was measured at selected time points (up to 5 days postinjection) within 3 dose levels: 0.1, 1.0, and 2.0 micrograms (micrograms). At each dose level, 3-9 animals were studied in each of 3 groups: animals receiving ip injection (group HT-29-15 ip), those receiving intravenous injection (group HT-29-15 iv), and those receiving isotype-matched control antibody via the intraportal route (group BL-3 ip). Significantly greater (P < 0.005) %id/g in tumor was seen in group HT-29-15 ip at all time points and dose levels compared to those in groups HT-29-15 iv or BL-3 ip. However, immediately after injection of mAb, there was no difference in tumor %id/g between groups HT-29-15 ip and HT-29-15 iv at the highest dose level. There was no increase in %id/g of mAb in normal liver and blood after ip injection compared to iv injection beyond day 1. Therefore ip injection resulted in higher tumor to liver and tumor to blood ratios compared to iv (P < 0.005). We conclude that delivery of mAb to hepatic metastases can be enhanced by selective injection; this has important implications in the design of future clinical trials utilizing radiolabeled mAb in the diagnosis and treatment of hepatic metastases.

Duke Scholars

Published In

J Surg Oncol

DOI

ISSN

0022-4790

Publication Date

October 1993

Volume

54

Issue

2

Start / End Page

71 / 77

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Portal Vein
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Liver Neoplasms, Experimental
  • Injections, Intravenous
  • Humans
 

Citation

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Rivoire, M. L., Yoshida, K., Voiglio, E. J., Divgi, C. R., Niedzwiecki, D., Chapman, D., … Sigurdson, E. R. (1993). Intraportal injection of monoclonal antibody in nude mice bearing hepatic metastases. J Surg Oncol, 54(2), 71–77. https://doi.org/10.1002/jso.2930540203
Rivoire, M. L., K. Yoshida, E. J. Voiglio, C. R. Divgi, D. Niedzwiecki, D. Chapman, A. M. Cohen, and E. R. Sigurdson. “Intraportal injection of monoclonal antibody in nude mice bearing hepatic metastases.J Surg Oncol 54, no. 2 (October 1993): 71–77. https://doi.org/10.1002/jso.2930540203.
Rivoire ML, Yoshida K, Voiglio EJ, Divgi CR, Niedzwiecki D, Chapman D, et al. Intraportal injection of monoclonal antibody in nude mice bearing hepatic metastases. J Surg Oncol. 1993 Oct;54(2):71–7.
Rivoire, M. L., et al. “Intraportal injection of monoclonal antibody in nude mice bearing hepatic metastases.J Surg Oncol, vol. 54, no. 2, Oct. 1993, pp. 71–77. Pubmed, doi:10.1002/jso.2930540203.
Rivoire ML, Yoshida K, Voiglio EJ, Divgi CR, Niedzwiecki D, Chapman D, Cohen AM, Sigurdson ER. Intraportal injection of monoclonal antibody in nude mice bearing hepatic metastases. J Surg Oncol. 1993 Oct;54(2):71–77.
Journal cover image

Published In

J Surg Oncol

DOI

ISSN

0022-4790

Publication Date

October 1993

Volume

54

Issue

2

Start / End Page

71 / 77

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Portal Vein
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Liver Neoplasms, Experimental
  • Injections, Intravenous
  • Humans