The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.
Journal Article (Journal Article)
The use of alternative 5' splice sites in the simian virus 40 early-transcription unit controls the ratio of large T to small t antigen during viral infection. To study the regulation of these alternative 5' splice sites, we made two mutants which improve the match of the large-T-antigen 5' splice site to the 5' splice site consensus sequence. Whether these mutants were assayed in vitro or in vivo, we found that the efficiency of large-T splicing is increased by improving the match of the large-T-antigen 5' splice site to the consensus. We conclude that the match of a 5' splice site is an important determinant of 5' splice site utilization and that the simian virus 40 large-T-antigen 5' splice site is almost certainly recognized by the U1 small nuclear RNA component of the U1 small nuclear ribonucleoprotein particle.
Full Text
Duke Authors
Cited Authors
- Zhuang, Y; Leung, H; Weiner, AM
Published Date
- August 1987
Published In
Volume / Issue
- 7 / 8
Start / End Page
- 3018 - 3020
PubMed ID
- 2823114
Pubmed Central ID
- PMC367927
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/mcb.7.8.3018-3020.1987
Language
- eng
Conference Location
- United States