The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.

Journal Article (Journal Article)

The use of alternative 5' splice sites in the simian virus 40 early-transcription unit controls the ratio of large T to small t antigen during viral infection. To study the regulation of these alternative 5' splice sites, we made two mutants which improve the match of the large-T-antigen 5' splice site to the 5' splice site consensus sequence. Whether these mutants were assayed in vitro or in vivo, we found that the efficiency of large-T splicing is increased by improving the match of the large-T-antigen 5' splice site to the consensus. We conclude that the match of a 5' splice site is an important determinant of 5' splice site utilization and that the simian virus 40 large-T-antigen 5' splice site is almost certainly recognized by the U1 small nuclear RNA component of the U1 small nuclear ribonucleoprotein particle.

Full Text

Duke Authors

Cited Authors

  • Zhuang, Y; Leung, H; Weiner, AM

Published Date

  • August 1987

Published In

Volume / Issue

  • 7 / 8

Start / End Page

  • 3018 - 3020

PubMed ID

  • 2823114

Pubmed Central ID

  • PMC367927

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.7.8.3018-3020.1987


  • eng

Conference Location

  • United States