A conserved transcriptional enhancer regulates RAG gene expression in developing B cells.

Published

Journal Article

Although expression of the RAG1 and RAG2 genes is essential for lymphocyte development, the mechanisms responsible for the lymphoid- and developmental stage-specific regulation of these genes are poorly understood. We have identified a novel, evolutionarily conserved transcriptional enhancer in the RAG locus, called Erag, which was essential for the expression of a chromosomal reporter gene driven by either RAG promoter. Targeted deletion of Erag in the mouse germline results in a partial block in B cell development associated with deficient V(D)J recombination, whereas T cell development appears unaffected. We found that E2A transcription factors bind to Erag in vivo and can transactivate Erag-dependent reporter constructs in cotransfected cell lines. These findings lead us to conclude that RAG transcription is regulated by distinct elements in developing B and T cells and that Erag is required for optimal levels of RAG expression in early B cell precursors but not in T cells.

Full Text

Duke Authors

Cited Authors

  • Hsu, L-Y; Lauring, J; Liang, H-E; Greenbaum, S; Cado, D; Zhuang, Y; Schlissel, MS

Published Date

  • July 2003

Published In

Volume / Issue

  • 19 / 1

Start / End Page

  • 105 - 117

PubMed ID

  • 12871643

Pubmed Central ID

  • 12871643

International Standard Serial Number (ISSN)

  • 1074-7613

Language

  • eng

Conference Location

  • United States