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A conserved transcriptional enhancer regulates RAG gene expression in developing B cells.

Publication ,  Journal Article
Hsu, L-Y; Lauring, J; Liang, H-E; Greenbaum, S; Cado, D; Zhuang, Y; Schlissel, MS
Published in: Immunity
July 2003

Although expression of the RAG1 and RAG2 genes is essential for lymphocyte development, the mechanisms responsible for the lymphoid- and developmental stage-specific regulation of these genes are poorly understood. We have identified a novel, evolutionarily conserved transcriptional enhancer in the RAG locus, called Erag, which was essential for the expression of a chromosomal reporter gene driven by either RAG promoter. Targeted deletion of Erag in the mouse germline results in a partial block in B cell development associated with deficient V(D)J recombination, whereas T cell development appears unaffected. We found that E2A transcription factors bind to Erag in vivo and can transactivate Erag-dependent reporter constructs in cotransfected cell lines. These findings lead us to conclude that RAG transcription is regulated by distinct elements in developing B and T cells and that Erag is required for optimal levels of RAG expression in early B cell precursors but not in T cells.

Duke Scholars

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

July 2003

Volume

19

Issue

1

Start / End Page

105 / 117

Location

United States

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Recombinases
  • Receptors, Antigen, B-Cell
  • Promoter Regions, Genetic
  • Nuclear Proteins
  • Molecular Sequence Data
  • Mice
  • Immunology
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hsu, L.-Y., Lauring, J., Liang, H.-E., Greenbaum, S., Cado, D., Zhuang, Y., & Schlissel, M. S. (2003). A conserved transcriptional enhancer regulates RAG gene expression in developing B cells. Immunity, 19(1), 105–117. https://doi.org/10.1016/s1074-7613(03)00181-x
Hsu, Lih-Yun, Josh Lauring, Hong-Erh Liang, Stephen Greenbaum, Dragana Cado, Yuan Zhuang, and Mark S. Schlissel. “A conserved transcriptional enhancer regulates RAG gene expression in developing B cells.Immunity 19, no. 1 (July 2003): 105–17. https://doi.org/10.1016/s1074-7613(03)00181-x.
Hsu L-Y, Lauring J, Liang H-E, Greenbaum S, Cado D, Zhuang Y, et al. A conserved transcriptional enhancer regulates RAG gene expression in developing B cells. Immunity. 2003 Jul;19(1):105–17.
Hsu, Lih-Yun, et al. “A conserved transcriptional enhancer regulates RAG gene expression in developing B cells.Immunity, vol. 19, no. 1, July 2003, pp. 105–17. Pubmed, doi:10.1016/s1074-7613(03)00181-x.
Hsu L-Y, Lauring J, Liang H-E, Greenbaum S, Cado D, Zhuang Y, Schlissel MS. A conserved transcriptional enhancer regulates RAG gene expression in developing B cells. Immunity. 2003 Jul;19(1):105–117.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

July 2003

Volume

19

Issue

1

Start / End Page

105 / 117

Location

United States

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Recombinases
  • Receptors, Antigen, B-Cell
  • Promoter Regions, Genetic
  • Nuclear Proteins
  • Molecular Sequence Data
  • Mice
  • Immunology
  • Humans