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Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit.

Publication ,  Journal Article
Xie, D; Pippen, AM; Odronic, SI; Annex, BH; Donatucci, CF
Published in: J Sex Med
March 2006

PURPOSE: We determined the effects of intracavernosal injection (ICI) of recombinant basic fibroblast growth factor (rbFGF) on corporal tissue in hypercholesterolemic rabbits. METHODS: Twenty New Zealand White rabbits were fed a 1% cholesterol diet for 6 weeks and were randomly divided into four groups. Group 1 (N = 5) received an ICI of phosphate buffered saline solution (PBS) once and again 3 weeks later. Group 2 (N = 4) received an ICI of 2.5 microg rbFGF once and PBS 3 weeks later. Group 3 (N = 6) received an ICI of 2.5 microg rbFGF once and again 3 weeks later. Group 4 (N = 5) received an ICI of 2.5 microg rbFGF once. All animals were maintained on the high cholesterol diet until sacrifice, 3 weeks after last injection. Strips of corporal tissue were submaximally contracted with norepinephrine, and dose-response curves were generated to evaluate endothelial-dependent (acetylcholine, ACH) and endothelial-independent (sodium nitroprusside, SNP) vasoreactivity. Protein levels of bFGF and vascular endothelial growth factor (VEGF) were assessed by enzyme-linked immunosorbent assay. Neuronal nitric oxide synthase (nNOS) protein and mRNA were detected by Western blot and semi-quantitative polymerase chain reaction, respectively. RESULTS: Vasoreactivity was improved by bFGF treatment as shown by higher ED50[-log(M)] of ACH and SNP in Groups 2, 3, and 4. The expression of bFGF protein, VEGF protein, nNOS protein, and mRNA were all increased after bFGF treatment. CONCLUSIONS: ICI of bFGF improved vasoreactivity in hypercholesterolemic rabbit corporal tissue, offering a new direction to explore for the treatment of erectile dysfunction.

Published In

J Sex Med

DOI

ISSN

1743-6095

Publication Date

March 2006

Volume

3

Issue

2

Start / End Page

223 / 232

Location

Netherlands

Related Subject Headings

  • Vasodilation
  • Vascular Endothelial Growth Factor A
  • Sodium Chloride
  • Random Allocation
  • Rabbits
  • Penis
  • Obstetrics & Reproductive Medicine
  • Muscle, Smooth
  • Muscle Relaxation
  • Male
 

Citation

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Xie, D., Pippen, A. M., Odronic, S. I., Annex, B. H., & Donatucci, C. F. (2006). Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit. J Sex Med, 3(2), 223–232. https://doi.org/10.1111/j.1743-6109.2005.00174.x
Xie, Donghua, Anne M. Pippen, Shelley I. Odronic, Brian H. Annex, and Craig F. Donatucci. “Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit.J Sex Med 3, no. 2 (March 2006): 223–32. https://doi.org/10.1111/j.1743-6109.2005.00174.x.
Xie D, Pippen AM, Odronic SI, Annex BH, Donatucci CF. Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit. J Sex Med. 2006 Mar;3(2):223–32.
Xie, Donghua, et al. “Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit.J Sex Med, vol. 3, no. 2, Mar. 2006, pp. 223–32. Pubmed, doi:10.1111/j.1743-6109.2005.00174.x.
Xie D, Pippen AM, Odronic SI, Annex BH, Donatucci CF. Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit. J Sex Med. 2006 Mar;3(2):223–232.
Journal cover image

Published In

J Sex Med

DOI

ISSN

1743-6095

Publication Date

March 2006

Volume

3

Issue

2

Start / End Page

223 / 232

Location

Netherlands

Related Subject Headings

  • Vasodilation
  • Vascular Endothelial Growth Factor A
  • Sodium Chloride
  • Random Allocation
  • Rabbits
  • Penis
  • Obstetrics & Reproductive Medicine
  • Muscle, Smooth
  • Muscle Relaxation
  • Male