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The effect of soluble complement receptor type 1 on hyperacute allograft rejection.

Publication ,  Journal Article
Pruitt, SK; Bollinger, RR
Published in: J Surg Res
April 1991

A major obstacle to successful organ transplantation in sensitized recipients is antibody-mediated hyperacute rejection. We hypothesized that human recombinant soluble complement receptor type 1 (sCR1), which inhibits activation of the complement cascade at multiple stages, would delay this process. Using a well-established model of hyperacute rejection, 21 Lewis rats each received three successive ACI rat skin grafts which resulted in high serum titers of ACI-specific antibodies. These hypersensitized Lewis rats then received heterotopic ACI cardiac allografts. Immediately prior to allograft reperfusion, sCR1 at 3 mg/kg (n = 11) or an equivalent volume of phosphate-buffered saline (PBS) (n = 10) was administered intravenously. Five minutes following allograft reperfusion, hemolytic complement activity was reduced by 63 +/- 2% (SEM) in the sCR1 group vs 25 +/- 3% in the PBS group (P less than 0.0001, Wilcoxon rank sum test (WRST)). Graft survival in the sCR1 group was prolonged to 32.0 +/- 4.47 hr vs 3.25 +/- 0.81 hr in the PBS group (P less than 0.0001, WRST). Serial histologic examination of allografts showed that sCR1 therapy prevented the early development of luminal platelet thrombi in the allograft coronary vessels. This study demonstrates that a single 3 mg/kg dose of sCR1 significantly prolongs ACI cardiac allograft survival in the hypersensitized Lewis rat recipient. Complement inactivation, mediated by sCR1, may prove useful for transplantation in sensitized recipients.

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Published In

J Surg Res

DOI

ISSN

0022-4804

Publication Date

April 1991

Volume

50

Issue

4

Start / End Page

350 / 355

Location

United States

Related Subject Headings

  • Surgery
  • Solubility
  • Receptors, Complement 3b
  • Receptors, Complement
  • Rats, Inbred Lew
  • Rats
  • Male
  • Isoantibodies
  • Heart Transplantation
  • Graft Survival
 

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Pruitt, S. K., & Bollinger, R. R. (1991). The effect of soluble complement receptor type 1 on hyperacute allograft rejection. J Surg Res, 50(4), 350–355. https://doi.org/10.1016/0022-4804(91)90202-w
Pruitt, S. K., and R. R. Bollinger. “The effect of soluble complement receptor type 1 on hyperacute allograft rejection.J Surg Res 50, no. 4 (April 1991): 350–55. https://doi.org/10.1016/0022-4804(91)90202-w.
Pruitt SK, Bollinger RR. The effect of soluble complement receptor type 1 on hyperacute allograft rejection. J Surg Res. 1991 Apr;50(4):350–5.
Pruitt, S. K., and R. R. Bollinger. “The effect of soluble complement receptor type 1 on hyperacute allograft rejection.J Surg Res, vol. 50, no. 4, Apr. 1991, pp. 350–55. Pubmed, doi:10.1016/0022-4804(91)90202-w.
Pruitt SK, Bollinger RR. The effect of soluble complement receptor type 1 on hyperacute allograft rejection. J Surg Res. 1991 Apr;50(4):350–355.
Journal cover image

Published In

J Surg Res

DOI

ISSN

0022-4804

Publication Date

April 1991

Volume

50

Issue

4

Start / End Page

350 / 355

Location

United States

Related Subject Headings

  • Surgery
  • Solubility
  • Receptors, Complement 3b
  • Receptors, Complement
  • Rats, Inbred Lew
  • Rats
  • Male
  • Isoantibodies
  • Heart Transplantation
  • Graft Survival