Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes.
We investigated the effects of human melanoma-specific cytotoxic T lymphocytes in treating experimental human melanoma hepatic metastases in a nude mouse model of adoptive immunotherapy. Hepatic metastases were generated by intrasplenic injection of 1.5 x 10(6) human melanoma cells. Three days after injection, animals received salt solution and interleukin 2 or interleukin 2 and cytotoxic T lymphocytes. Twenty-four of 25 control animals had developed multiple tumor nodules in the liver; 11 of 13 animals receiving only interleukin 2 also had significant tumor burdens. In striking contrast, 17 of 18 animals receiving cytotoxic T lymphocytes and interleukin 2 had no gross or histologic evidence of tumors. The remaining animal had a 2-mm nodule. Human tumor-specific cytotoxic T lymphocytes are effective in vivo in a model of adoptive immunotherapy and may prove useful in adoptive immunotherapy of humans with metastatic melanoma.
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- Tumor Cells, Cultured
- T-Lymphocytes, Cytotoxic
- Surgery
- Neoplasm Transplantation
- Mice, Nude
- Mice
- Melanoma
- Male
- Liver Neoplasms
- Interleukin-2
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Cells, Cultured
- T-Lymphocytes, Cytotoxic
- Surgery
- Neoplasm Transplantation
- Mice, Nude
- Mice
- Melanoma
- Male
- Liver Neoplasms
- Interleukin-2