Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes.

Journal Article (Journal Article)

We investigated the effects of human melanoma-specific cytotoxic T lymphocytes in treating experimental human melanoma hepatic metastases in a nude mouse model of adoptive immunotherapy. Hepatic metastases were generated by intrasplenic injection of 1.5 x 10(6) human melanoma cells. Three days after injection, animals received salt solution and interleukin 2 or interleukin 2 and cytotoxic T lymphocytes. Twenty-four of 25 control animals had developed multiple tumor nodules in the liver; 11 of 13 animals receiving only interleukin 2 also had significant tumor burdens. In striking contrast, 17 of 18 animals receiving cytotoxic T lymphocytes and interleukin 2 had no gross or histologic evidence of tumors. The remaining animal had a 2-mm nodule. Human tumor-specific cytotoxic T lymphocytes are effective in vivo in a model of adoptive immunotherapy and may prove useful in adoptive immunotherapy of humans with metastatic melanoma.

Full Text

Duke Authors

Cited Authors

  • Crowley, NJ; Vervaert, CE; Seigler, HF

Published Date

  • April 1991

Published In

Volume / Issue

  • 126 / 4

Start / End Page

  • 433 - 437

PubMed ID

  • 2009057

International Standard Serial Number (ISSN)

  • 0004-0010

Digital Object Identifier (DOI)

  • 10.1001/archsurg.1991.01410280031003


  • eng

Conference Location

  • United States