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Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes.

Publication ,  Journal Article
Crowley, NJ; Vervaert, CE; Seigler, HF
Published in: Arch Surg
April 1991

We investigated the effects of human melanoma-specific cytotoxic T lymphocytes in treating experimental human melanoma hepatic metastases in a nude mouse model of adoptive immunotherapy. Hepatic metastases were generated by intrasplenic injection of 1.5 x 10(6) human melanoma cells. Three days after injection, animals received salt solution and interleukin 2 or interleukin 2 and cytotoxic T lymphocytes. Twenty-four of 25 control animals had developed multiple tumor nodules in the liver; 11 of 13 animals receiving only interleukin 2 also had significant tumor burdens. In striking contrast, 17 of 18 animals receiving cytotoxic T lymphocytes and interleukin 2 had no gross or histologic evidence of tumors. The remaining animal had a 2-mm nodule. Human tumor-specific cytotoxic T lymphocytes are effective in vivo in a model of adoptive immunotherapy and may prove useful in adoptive immunotherapy of humans with metastatic melanoma.

Duke Scholars

Published In

Arch Surg

DOI

ISSN

0004-0010

Publication Date

April 1991

Volume

126

Issue

4

Start / End Page

433 / 437

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Melanoma
  • Male
  • Liver Neoplasms
  • Interleukin-2
 

Citation

APA
Chicago
ICMJE
MLA
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Crowley, N. J., Vervaert, C. E., & Seigler, H. F. (1991). Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes. Arch Surg, 126(4), 433–437. https://doi.org/10.1001/archsurg.1991.01410280031003
Crowley, N. J., C. E. Vervaert, and H. F. Seigler. “Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes.Arch Surg 126, no. 4 (April 1991): 433–37. https://doi.org/10.1001/archsurg.1991.01410280031003.
Crowley NJ, Vervaert CE, Seigler HF. Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes. Arch Surg. 1991 Apr;126(4):433–7.
Crowley, N. J., et al. “Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes.Arch Surg, vol. 126, no. 4, Apr. 1991, pp. 433–37. Pubmed, doi:10.1001/archsurg.1991.01410280031003.
Crowley NJ, Vervaert CE, Seigler HF. Treatment of human melanoma hepatic metastases in nude mice with human cytotoxic T lymphocytes. Arch Surg. 1991 Apr;126(4):433–437.

Published In

Arch Surg

DOI

ISSN

0004-0010

Publication Date

April 1991

Volume

126

Issue

4

Start / End Page

433 / 437

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Melanoma
  • Male
  • Liver Neoplasms
  • Interleukin-2