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Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody.

Publication ,  Journal Article
Abdel-Wahab, Z; Darrow, T; Vervaert, CE; Crowley, NJ; Seigler, HF
Published in: Mol Biother
September 1991

Murine, antihuman melanoma cell monoclonal antibody (mAb) 16.C8 was generated by fusing the murine myeloma cell line P3X63/Ag8.653 with splenocytes from a nude mouse bearing a human melanoma xenograft, after reconstitution with splenocytes from syngeneic immunocompetent BALB/c mice. The antibody reacted strongly with fresh human melanoma cells and exhibited preferential reactivity with established human melanoma and neuroectodermal tumor cell lines. Electrophoresis and Western blotting experiments indicated that 16.C8 is directed against a sialoglycoprotein antigen with a molecular weight of 110-120 kDa. mAb 16.C8 mediated lysis of melanoma cells in vitro in antibody-dependent cellular cytotoxicity assays using human mononuclear effector cells isolated from normal volunteers or malignant melanoma patients. In addition, the administration of mAb 16.C8 to nude mice bearing established human melanoma lung and liver metastases resulted in significant inhibition of tumor growth as shown by gross and histologic examination. In contrast, animals treated with Hanks' balanced salt solution or nonspecific immunoglobulin exhibited a large tumor burden. These results suggest that mAb 16.C8 may be of value in treatment of metastatic melanoma in humans.

Duke Scholars

Published In

Mol Biother

ISSN

0952-8172

Publication Date

September 1991

Volume

3

Issue

3

Start / End Page

163 / 169

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Melanoma
  • Immunotherapy
  • Humans
  • Blotting, Western
  • Antibody-Dependent Cell Cytotoxicity
  • Antibody Specificity
 

Citation

APA
Chicago
ICMJE
MLA
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Abdel-Wahab, Z., Darrow, T., Vervaert, C. E., Crowley, N. J., & Seigler, H. F. (1991). Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody. Mol Biother, 3(3), 163–169.
Abdel-Wahab, Z., T. Darrow, C. E. Vervaert, N. J. Crowley, and H. F. Seigler. “Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody.Mol Biother 3, no. 3 (September 1991): 163–69.
Abdel-Wahab Z, Darrow T, Vervaert CE, Crowley NJ, Seigler HF. Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody. Mol Biother. 1991 Sep;3(3):163–9.
Abdel-Wahab, Z., et al. “Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody.Mol Biother, vol. 3, no. 3, Sept. 1991, pp. 163–69.
Abdel-Wahab Z, Darrow T, Vervaert CE, Crowley NJ, Seigler HF. Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody. Mol Biother. 1991 Sep;3(3):163–169.

Published In

Mol Biother

ISSN

0952-8172

Publication Date

September 1991

Volume

3

Issue

3

Start / End Page

163 / 169

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Melanoma
  • Immunotherapy
  • Humans
  • Blotting, Western
  • Antibody-Dependent Cell Cytotoxicity
  • Antibody Specificity